|Tested species reactivity||Human, Mouse, Xenopus|
|Published species reactivity||Rabbit|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant His 6 tagged-SMN protein.|
|Contains||0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
MA1-5878 detects SMN in human, mouse and xenopus samples.
MA1-5878 has been successfully used in Western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin), flow cytometry, and ELISA procedures. MA1-5878 detects a band of approximately 42kDa.
The MA1-5878 immunogen is purified recombinant His 6 tagged-SMN protein (human).
This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. The telomeric and centromeric copies of this gene are nearly identical and encode the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Coupled in vitro import of U snRNPs and SMN, the spinal muscular atrophy protein.
MA1-5878 was used in immunoprecipitation and western blot to study the coupled nuclear import of U snRNPs and the survival of motor neurons protein
|Narayanan U,Achsel T,Lührmann R,Matera AG||Molecular cell (16:223)||2004|