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SMS Antibody (MA5-44855) in ICC/IF
Immunocytochemistry analysis of Spermine synthase in HeLa cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 , permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Samples were incubated in Spermine synthase Monoclonal antibody (Product # MA5-44855) using a dilution of 1:50 in 2% negative goat serum overnight at 4 followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at a dilution of 1:1,000. Nuclear DNA was label... View More
Product Details
MA5-44855
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE61-85
Immunogen
Synthetic peptide within human Spermine synthase aa 100-150/366.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2931311
Target Information
Catalyzes the production of spermine from spermidine and decarboxylated S-adenosylmethionine (dcSAM).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Spermidine aminopropyltransferase; Spermine synthase; SPMSY
Gene Aliases: AI427066; Gy; gyro; MRSR; SMS; SpmST; SPMSY; SpS; SRS
UniProt ID: (Human) P52788, (Mouse) P97355
Entrez Gene ID: (Human) 6611, (Mouse) 20603, (Rat) 363469
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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