Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunofluorescent analysis of SUMO 3 in U2OS cells using a SUMO 3 recombinant rabbit monoclonal antibody (Product # 700186) at a dilution of 5.0ug/ml followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000 (left) and in the presence of the immunogenic phosphopeptide (right).
|Tested species reactivity||Rat , Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 91-103 of P55854.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunocytochemistry (ICC)||4-6 ug/ml|
|Immunofluorescence (IF)||1-5 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||4-6 ug/ml|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with chimpanzee and Rhesus monkey based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Small ubiquitin-related modifier proteins are members of the ubiquitin-like protein family. SUMO-3 regulates Amyloid beta generation and may be critical in the onset or progression of Alzheimer's disease. SUMO-2/3 forms poly-. SUMO-1, 2, and 3 proteins localize to the nuclear membrane, nuclear bodies, and cytoplasm, respectively. SUMOylation is essential for mammalian cell cycle regulation, SUMO-2/3 localizes to centromeres and condensed chromosomes where binds CENP-E.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
SUMO-3, SMT3H1, Smt3h1, D10Ertd345e, SMT3A, Smt3B, 2810014B19Rik
SMT3 (supressor of mif two, 3) homolog 1, small ubiquitin-related modifier 3, ubiquitin-like protein SMT3A, SMT3 homolog 1, SMT3 suppressor of mif two 3 homolog 1, SMT3 suppressor of mif two 3 homolog 3, SUMO-2, ubiquitin-like protein SMT3B, SUMO-3, SMT3A, SMT3H1, Smt3B