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Immunoprecipitation analysis of Anti-SMYD3 Recombinant Mouse Monoclonal Antibody (Cat. no. 730061). Immunoprecipitation analysis was performed on HEK293 cells over expressing flag tagged SMYD3 protein. Western blot using anti-flag antibody was performed on the raw lysate (lane 1), and samples immunoprecipitated with Anti-SMYD3 Recombinant Mouse Monoclonal Antibody (lane 2).
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||2-428 of 428 of SMYD3 Protein.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
SMYD3 is a histone methyltransferase that plays a role in transcriptional regulation as a member of an RNA polymerase complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.
730061 was used in immunoprecipitation to describe a mass spectrometry-based method for scoring immunoprecipitation antibody quality
|Marcon E,Jain H,Bhattacharya A,Guo H,Phanse S,Pu S,Byram G,Collins BC,Dowdell E,Fenner M,Guo X,Hutchinson A,Kennedy JJ,Krastins B,Larsen B,Lin ZY,Lopez MF,Loppnau P,Miersch S,Nguyen T,Olsen JB,Paduch M,Ravichandran M,Seitova A,Vadali G,Vogelsang MS,Whiteaker JR,Zhong G,Zhong N,Zhao L,Aebersold R,Arrowsmith CH,Emili A,Frappier L,Gingras AC,Gstaiger M,Paulovich AG,Koide S,Kossiakoff AA,Sidhu SS,Wodak SJ,Gräslund S,Greenblatt JF,Edwards AM||Nature methods (12:725)||2015|
1; bA74P14.1; bA74P14.1 (novel protein); KMT3E; MYND domain containing 1; SET and MYND domain-containing protein 3; subfamily 3A (MYND domain containing); zinc finger; zinc finger MYND domain-containing protein 1; zinc finger protein; zinc finger protein, subfamily 3A (MYND domain containing), 1; zinc finger, MYND domain containing 1; ZMYND1; ZNFN3A1
bA74P14.1; KMT3E; SMYD3; ZMYND1; ZNFN3A1