Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 4
SNAI1 Antibody (600-401-MM5) in IHC
Immunohistochemistry of Rabbit Anti-SNAI1 Antibody. Tissue: Human Lung Tissue. Fixative: 10% formalin. Antigen Retrieval: HIER using citrate buffer for 20 minutes. Primary Antibody: Anti-SNAI1 at 1:300 for 30 minutes at RT. Secondary Antibody: Anti-Rabbit Poly-HRP-IgG Ready-to-Use for 8 minutes at RT. Counterstain: Hematoxylin. Developing: DAB. Staining: Shows strong nuclear and cytoplasmic staining of pneumocytes, macrophages, and bronchial epithelium. Weaker staining is also noted in smooth muscle and vascular endothelium.
Product Details
600-401-MM5
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-SNAI1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human SNAI1 conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate
Unconjugated
Form
Liquid
Concentration
1.03 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human SNAI1. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human and mouse.
Target Information
Zinc finger protein SNAI1 (SNAIL)is a zinc finger transcriptional repressor which down regulates the expression of the ectodermal gene within the mesoderm and functions to form the mesoderm and neural crest. It is reported that SNAI1 acts as an important factor of tumor invasion as evidenced by its role in E-cadherin down-regulation and induction of epithelial-mesenchymal transition (EMT). In human breast cancer, the expression of SNAI1 and/or the homologous SNAI2 (Slug) has been associated with E-cadherin repression, local or distant metastasis, tumor recurrence, or poor prognosis in different tumor series. It is also reported that Snail 1 protein in the stromamay be anew putative prognosis marker for colon tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Protein sna; Protein snail homolog 1; Sna protein; snai; snail 1; snail 1 homolog; snail 1 zinc finger protein; snail 1, zinc finger protein; snail family zinc finger 1; snail homolog 1; Zinc finger protein SNAI1
Gene Aliases: AI194338; dJ710H13.1; SLUGH2; SNA; Sna1; SNAH; SNAI1; SNAIL; SNAIL1
UniProt ID: (Human) O95863, (Mouse) Q02085
Entrez Gene ID: (Human) 6615, (Mouse) 20613
Molecular Function:
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
osteoblast differentiation
mesoderm formation
epithelial to mesenchymal transition
epithelial to mesenchymal transition involved in endocardial cushion formation
positive regulation of epithelial to mesenchymal transition
negative regulation of vitamin D biosynthetic process
cell migration
positive regulation of cell migration
hair follicle morphogenesis
negative regulation of DNA damage response, signal transduction by p53 class mediator
positive regulation of transcription, DNA-templated
palate development
cartilage morphogenesis
trophoblast giant cell differentiation
negative regulation of cell differentiation involved in embryonic placenta development
left/right pattern formation
Notch signaling involved in heart development
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage
regulation of bicellular tight junction assembly
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
multicellular organism development
mesoderm development
negative regulation of transcription, DNA-templated
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
protein binding
kinase binding
metal ion binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
nucleic acid binding
DNA binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support