Western blot analysis of SNAI1 in human MCF7 cell lysate using 1) prebleed at 1:5000 dilution and 2) a SNAI1/Snail polyclonal antibody (Product # PA5-23472) at 5 ug/ml, and 3) in mouse D3 cell lysate using a SNAI1/Snail polyclonal antibody (Product # PA5-23472) at 3 ug/ml.
|Tested species reactivity||Dog, Horse, Human, Mouse|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (87 ELTSLSDEDSGKGSQPPS 104) of SNAI1/Snail protein.|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||3-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Zinc finger protein SNAI1 is a zinc finger transcriptional repressor which down regulates the expression of ectodermal gene within the mesoderm and thus functions to form the mesoderm and neural crest. It is reported that SNAI1 acts as an important factor of tumor invasion as evidenced by its role in E-cadherin down-regulation and induction of epithelial-mesenchymal transition (EMT). In human breast cancer, the expression of SNAI1 and/or the homologous SNAI2 (Slug) has been associated with E-cadherin repression, local or distant metastasis, tumor recurrence, or poor prognosis in different tumor series. It is also reported that Snail 1 protein in the stroma is a new putative prognosis marker for colon tumors. In recent studies SNAI1 was found to be potential target for Proteasome degradation by hypoxia-controlled FBXL14 ubiquitin ligase targets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The Vitamin D Analog, MART-10, Attenuates Triple Negative Breast Cancer Cells Metastatic Potential.
PA5-23472 was used in western blot to characterize attenuation of triple negative breast cancer cells metastatic potential by the vitamin D analog, MART-10
|Chiang KC,Yeh TS,Chen SC,Pang JH,Yeh CN,Hsu JT,Chen LW,Kuo SF,Takano M,Kittaka A,Chen TC,Sun CC,Juang HH||International journal of molecular sciences (17:null)||2016|
MART-10, the vitamin D analog, is a potent drug to inhibit anaplastic thyroid cancer cell metastatic potential.
PA5-23472 was used in western blot to examine the effects of vitamin D on anaplastic thyroid cancer
|Chiang KC,Kuo SF,Chen CH,Ng S,Lin SF,Yeh CN,Chen LW,Takano M,Chen TC,Juang HH,Kittaka A,Lin JD,Pang JH||Cancer letters (369:76)||2015|