|Tested species reactivity||Bovine, Mouse, Rat, Zebrafish|
|Published species reactivity||Rat, Mouse, Chicken, Guinea pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide:(C)- A(195) N Q R A T K M L G S G(206)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg x 10^6 cells|
|Western Blot (WB)||0.5-2 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-740 detects synaptosomal associated protein 25 (SNAP-25) from bovine, mouse, rat and zebra fish (Danio rerio) samples.
PA1-740 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a 25 kDa protein representing SNAP-25 in whole rat brain, mouse brain and zebra fish extracts. Immunofluorescence staining of SNAP-25 in cultured rat hippocampal cells with PA1-740 yields a pattern consistent with cytoplasmic and plasma membrane staining.
PA1-740 immunizing peptide corresponds to the C-terminal amino acid residues 195-206 of mouse SNAP-25. This sequence is completely conserved between mouse, rat, human, chicken, and goldfish. PA1-740 immunizing peptide (Cat. # PEP-032) is available for use in neutralization and control experiments.
Synaptosomal associated protein of 25 kDa (SNAP-25) is a soluble protein which plays a key role in vesicle membrane fusion events with the plasma membrane. SNAP-25, synaptobrevin, and syntaxin form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins NSF and alpha-SNAP. Functional interactions exist between SNAP-25, syntaxin, and the synaptic protein interaction site on voltage-sensitive L- and N-type calcium channels. SNAP-25 has been shown to be required for synaptic vesicle fusion with the plasma membrane during release of nerve growth factor (NGF). Regulated exocytosis of cortical granule secretion in fertilized eggs and membrane fusion events in neurons and endocrine cells is mediated by SNAP-25 in a calcium-dependent mechanism. SNAP-25 protein levels have been shown to be elevated in prolactinoma and growth hormone (GH)/prolactin (PRL) tumor cells while reduced SNAP-25 protein expression has been observed between schizophrenic and normal hippocampal cells. Altered patterns of SNAP-25 expression have also been observed in the inferior temporal cortex and prefrontal association cortex between normal brains and brains from individuals affected with schizophrenia. SNAP-25 accumulation due to cytoskeletal dysfunction is also observed in the swollen axons of the white matter of individuals with severe Alzheimer and quote;s dementia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mouse taste cells with G protein-coupled taste receptors lack voltage-gated calcium channels and SNAP-25.
PA1-740 was used in blocking/activating experiment to investigate the interaction between synapses with the nervous system and cells sensing various tastes
|Clapp TR,Medler KF,Damak S,Margolskee RF,Kinnamon SC||BMC biology (4:null)||2006|
|Guinea pig||Not Cited||
Most peptide-containing sensory neurons lack proteins for exocytotic release and vesicular transport of glutamate.
PA1-740 was used in immunohistochemistry to investigate the expression of proteins involved in vesicular uptake and exocytotic release of glutamate in peptide-containing sensory neurons of guinea pigs, mice and toads
|Morris JL,König P,Shimizu T,Jobling P,Gibbins IL||The Journal of comparative neurology (483:1)||2005|
Association of beta-catenin with the alpha-subunit of neuronal large-conductance Ca2+-activated K+ channels.
PA1-740 was used in western blot to study the association of beta-catenin with the alpha-subunit of neuronal large-conductance calcium-activated potassium channels.
|Lesage F,Hibino H,Hudspeth AJ||Proceedings of the National Academy of Sciences of the United States of America (101:671)||2004|
Accumulation of synaptosomal-associated protein of 25 kDa (SNAP-25) and other proteins associated with the secretory pathway in GH4C1 cells upon treatment with estradiol, insulin, and epidermal growth factor.
PA1-740 was used in immunocytochemistry to determine the mechanism for the effects of estradiol, insulin, and epidermal growth factor on SNAP-25 accumulation in GH4C1 cells.
|Lee MS,Zhu YL,Sun Z,Rhee H,Jeromin A,Roder J,Dannies PS||Endocrinology (141:3485)||2000|