Invitrogen

SNX27 Monoclonal Antibody (1C6)

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SNX27 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of SNX27 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: SNX27 Monoclonal Antibody (1C6) (Product # MA5-27854) diluted at 1:200. Blue: Hoechst 33342 staining.

SNX27 Antibody in Western Blot (WB) — Western blot analysis of SNX27 in whole cell lysate using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with SNX27 monoclonal antibody (Product # MA5-27854) using a dilution of 1:500.

Product Details

MA5-27854

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	1C6
Immunogen	Human SNX27
Conjugate	Unconjugated
Form	Liquid
Concentration	1.37 mg/mL
Purification	Protein G
Storage buffer	PBS
Contains	no preservative
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2735367

Product Specific Information

Positive Control: 293T, A431, HeLa, HepG2

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

SNX27 encodes a member of the sorting nexin family, a diverse group of cytoplasmic and membrane-associated proteins involved in endocytosis of plasma membrane receptors and protein trafficking through these compartments. All members of this protein family contain a phosphoinositide binding domain (PX domain). A highly similar protein in mouse is responsible for the specific recruitment of an isoform of serotonin 5-hydroxytryptamine 4 receptor into early endosomes, suggesting the analogous role for the human protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.