Immunohistochemistry analysis of SOCS-1 showing staining in the nucleus and weak staining in the cytoplasm of paraffin-embedded human spleen tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a SOCS-1 Mouse Monoclonal Antibody (37-4100) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant protein derived from the N-terminal region of the mouse SOCS-1 (Suppressor of Cytokine Signaling-1, also known as SSI-1) protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The Suppressor of cytokine signaling (SOCS) and cytokine-inducible SH2 proteins are a family of intracellular proteins which regulate the immune cell responses to cytokines. SOCS1 acts to suppress dendritic cell (DC) as well as T cell hyperactivation following cytokine signaling by inhibiting JAK tyrosine kinase, a kinase necessary for type I and II cytokine receptors to initiate signaling, by directly binding to the catalytic domain of the kinase. SOCS1 also possesses E3 ubiquitin protein ligase activity that results in the polyubiquitination of its target proteins and subsequent degradation by the proteosome. It is through this method that SOCS1 negatively regulates signaling by Toll-like receptors TLR2 and TLR4 by mediating the degradation of the TLR signaling adaptor protein TIRAP.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
||The SOCS box of suppressor of cytokine signaling-1 is important for inhibition of cytokine action in vivo.||Zhang JG,Metcalf D,Rakar S,Asimakis M,Greenhalgh CJ,Willson TA,Starr R,Nicholson SE,Carter W,Alexander WS,Hilton DJ,Nicola NA||Proceedings of the National Academy of Sciences of the United States of America (98:13261)||2001|
|Mouse||Not Cited||The SOCS box of suppressor of cytokine signaling-1 is important for inhibition of cytokine action in vivo.||Zhang JG,Metcalf D,Rakar S,Asimakis M,Greenhalgh CJ,Willson TA,Starr R,Nicholson SE,Carter W,Alexander WS,Hilton DJ,Nicola NA||Proceedings of the National Academy of Sciences of the United States of America (98:13261)||2001|