Immunofluorescent analysis of SOX2 using anti-SOX2 polyclonal antibody (Product# PA1-094) shows specific expression in human embryonal carcinoma NTERA-2 cells (shown in green) but not in negative control HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product #37525) for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing SOX2 (Product# PA1-094), at a dilution of 1:200 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody (Product# 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DY-547 phalloidin, nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
|Tested species reactivity||Fish, Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Full-length human recombinant protein expressed in bacteria|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100|
|Immunohistochemistry (Frozen) (IHC (F))||1:50-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:500|
|Immunoprecipitation (IP)||5µg, 1:1000|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
PA1-094 detects SOX2 in human and mouse samples and has been successfully used in Western blot, immunofluorescence, IHC (P), FACS and immunoprecipitation applications. Western blot analysis of PA1-094 specifically detects SOX2 protein at ~36 kDa in the nucleus of human and mouse embryonal carcinoma and iPS cells. Upon overexposure of western blots, additional higher and lower molecular weight bands may appear. This antibody has also been successfully used in IHC to detect SOX2 in frozen sections of Knifefish brain samples.
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Aldh1 Expression and Activity Increase During Tumor Evolution in Sarcoma Cancer Stem Cell Populations.
PA1-094 was used in immunocytochemistry and western blot to characterize an increase of Aldh1 activity and expression during tumor evolution in sarcoma cancer stem cell populations
|Martinez-Cruzado L,Tornin J,Santos L,Rodriguez A,García-Castro J,Morís F,Rodriguez R||Scientific reports (6:null)||2016|