|Tested species reactivity||Human, Mouse, Sheep|
|Published species reactivity||Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide made to the N-terminal region of human SOX2 protein (within residues 1-100). [Swiss-Prot# P48431]|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:125-1:250|
|Western Blot (WB)||0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The target sequence has 92% sequence homology with chicken, 86% sequence homology with xenopus, and 71% sequence homology with zebrafish.
Suggested positive control: antigen standard for SOX2 (transient overexpression lysate), mouse brain lysate, mouse brain protein, uterual endometrial glands.
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
SETD7 Regulates the Differentiation of Human Embryonic Stem Cells.
PA1-16968 was used in immunohistochemistry to study how differentiation of human embryonic stem cells is regulated by SETD7
|Castaño J,Morera C,Sesé B,Boue S,Bonet-Costa C,Martí M,Roque A,Jordan A,Barrero MJ||PloS one (11:null)||2016|
Expansion and differentiation of germline-derived pluripotent stem cells on biomaterials.
PA1-16968 was used in immunocytochemistry to study biomaterials that maintain self-renewal and differentiation potential and those that promote the differentiation of germline-derived pluripotent stem cells
|Hoss M,¿ari¿ T,Denecke B,Peinkofer G,Bovi M,Groll J,Ko K,Salber J,Halbach M,Schöler HR,Zenke M,Neuss S||Tissue engineering. Part A (19:1067)||2013|
Human stem/progenitor cells from bone marrow enhance glial differentiation of rat neural stem cells: a role for transforming growth factor ß and Notch signaling.
PA1-16968 was used in immunocytochemistry to investigate the interaction between human stem/progenitor cells and rat neural stem cells in coculture
|Robinson AP,Foraker JE,Ylostalo J,Prockop DJ||Stem cells and development (20:289)||2011|
Characterization of pluripotent stem cells.
PA1-16968 was used in immunocytochemistry to describe all the immunodetection procedures needed to characterize mouse and human stem cell lines in different circumstances.
|Martí M,Mulero L,Pardo C,Morera C,Carrió M,Laricchia-Robbio L,Esteban CR,Izpisua Belmonte JC||Nature protocols (8:223)||2013|