|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein encoding aa 316-510 of human SOX-9 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is testis tissue.
SOX-9 is a member of the family of SOX (Sry-type high mobility group box) gene that was first identified on the basis of region with high homology to that of SRY (Sex-determining region Y). It is a transcription factor with a high mobility group DNA-binding domain that is expressed in all prechondrocytic and chondrocytic cells during embryonic development in a pattern that close parallels that of the gene for type II collagen. Phosphorylation of SOX-9 by PKA increases its DNA binding and transcriptional activity. SOX-9 regulates expression of chondrocyte phenotype related genes. IL-1 and TNF-a can cause a marked and rapid decrease in the levels of SOX-9 mRNA and/or protein in chondrocytes, thus strongly inhibiting the expression of genes for cartilage extracellular matrix proteins. The down-regulation of SOX-9 may have a crucial role in inhibiting expression of the cartilage phenotype in inflammatory joint diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.