Invitrogen

SOX10 Monoclonal Antibody (20B7), eBioscience™

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SOX10 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of SOX10 was performed using 70% confluent log phase A-375 cells treated with SOX10 polyclonal antibody (Product # 14-5923-80). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. cells were labeled with SOX10 polyclonal antibody (Product # PA5-40697) at 5µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

SOX10 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of formalin-fixed paraffin embedded human cerebellum stained with 10 µg/mL of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (left) or 10 µg/mL of Anti-Sox10 Purified (right) followed by Anti-Mouse IgG Biotin, Streptavidin HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.

SOX10 Antibody in Western Blot (WB) — Western blot was performed using Anti-SOX10 Monoclonal Antibody (20B7), eBioscience™ (Product # 14-5923-80) and a 58 kDa band corresponding to SOX10 was observed in the cell line tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of A-375 (Lane 1) was electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1ug/ml) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Product Details

14-5923-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/ml

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

10 µg/mL

Immunocytochemistry (ICC/IF)

5 - 10 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

20B7

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_2572892

Product Specific Information

Description: The monoclonal antibody 20B7 recognizes Sox10, is a member of the Sox superfamily (SRY-related HMG-box) that contain a DNA binding domain (high-mobility group domain) and functions as a transcription factor. Initially, Sox10 expression is localized to the developing neural crest and peripheral nervous system, but later is expressed in glial progenitors and mature oligodendrocytes. Sox10 is involved in stem cell maintenance, melanocyte differentiation, as well as central and peripheral gliogenesis and neurogenesis. Sox10 regulation of downstream proteins such as MASH1, PHOX2B, PDGF-alpha, and MITF is dependent on expression level. Sox10 is also expressed in multipotent vascular stem cells, melanomas, and gliomas.

The 20B7 antibody is reported to recognize human and rat Sox 10, and based on homology should recognize mouse Sox10.

Applications Reported: This 20B7 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.

Applications Tested: This 20B7 antibody has been tested by immunocytochemistry of formaldehyde fixed and permeabilized rat cells. This 20B7 antibody has also been tested by immunohistochemistry of human formalin-fixed paraffin embedded tissue sections using low pH antigen retreival and can be used at less than or equal to 10 µg/mL for both applications. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. It acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Early embryonic development and spatiotemporal localization of mammalian primordial germ cell-associated proteins in the basal rodent Lagostomus maximus.

Scientific reports

Leopardo NP,Vitullo AD

14-5923 was used in Immunohistochemistry to investigate the embryonic development and the spatiotemporal localisation of primordial germ cell-associated proteins in the basal Hystricognathi rodent, Lagostomus maximus.

Tue Apr 04 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: dominant megacolon; dominant megacolon, mouse, human homolog of; MGC15649; Protein SOX-21; SRY; SRY (sex determining region Y)-box 10; SRY box 10; SRY-box containing gene 10; SRY-related HMG-box gene 10; Transcription factor SOX-10; Transcription factor SOX-M

Gene Aliases: DOM; PCWH; Sox-10; SOX10; Sox21; WS2E; WS4; WS4C

UniProt ID: (Human) P56693, (Rat) O55170, (Mouse) Q04888

Entrez Gene ID: (Human) 6663, (Rat) 29361, (Mouse) 20665

Function(s)