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Immunofluorescent analysis of Sox2 (green) in H9 embryonic stem cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a Sox2 monoclonal antibody (Product # MA1-014) at a dilution of 1:200 overnight at 4°C, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification.
|Tested species reactivity||Dog, Fish, Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Full-length human recombinant protein expressed in bacteria|
|Storage buffer||PBS with 30% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1-3 ul|
|Flow Cytometry (Flow)||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Immunoprecipitation (IP)||5 µg|
|Western Blot (WB)||1:1000 - 1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Western blot analysis of MA1-014 specifically detects SOX2 protein at ~36 kDa in the nucleus of human and mouse embryonal carcinoma and iPS cells.
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Role of stress-activated OCT4A in the cell fate decisions of embryonal carcinoma cells treated with etoposide.
MA1-014 was used in western blot to elucidate the mechanism of p53-dependent upregulation of OCT4A and p21Cip1.
|Huna A,Salmina K,Erenpreisa J,Vazquez-Martin A,Krigerts J,Inashkina I,Gerashchenko BI,Townsend PA,Cragg MS,Jackson TR||Cell cycle (Georgetown, Tex.) (14:2969)||2015|
ANOP3; MCOPS3; sex determining region Y box 2; sex determining region Y-box 2; SRY (sex determining region Y)-box 2; SRY box 2; SRY-box containing gene 2; SRY-related HMG-box gene 2; transcription factor SOX-2; transcription factor SOX2
ANOP3; lcc; MCOPS3; Sox-2; SOX2; ysb