Western blot analysis of SOX2 was performed by loading 50ug of NCCIT and negative control HeLa cell lysates per well onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane. The membrane was blocked, and probed with an HRP-conjugated SOX2 monoclonal antibody (Product # MA1-014-HRP) at a dilution of 1:1000 overnight at 4C. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Full-length human recombinant protein expressed in bacteria|
|Storage buffer||proprietary buffer with proprietary stabilizer|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:250-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-014-HRP has been successfully used in Western blot with human and mouse samples. Western blot analysis of MA1-014-HRP specifically detects SOX2 protein at ~36 kDa in the nucleus of embryonal carcinoma cells.
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
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