Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is testis tissue.
SOX-9 is a member of the family of SOX (Sry-type high mobility group box) gene that was first identified on the basis of region with high homology to that of SRY (Sex-determining region Y). It is a transcription factor with a high mobility group DNA-binding domain that is expressed in all prechondrocytic and chondrocytic cells during embryonic development in a pattern that close parallels that of the gene for type II collagen. Phosphorylation of SOX-9 by PKA increases its DNA binding and transcriptional activity. SOX-9 regulates expression of chondrocyte phenotype related genes. IL-1 and TNF-a can cause a marked and rapid decrease in the levels of SOX-9 mRNA and/or protein in chondrocytes, thus strongly inhibiting the expression of genes for cartilage extracellular matrix proteins. The down-regulation of SOX-9 may have a crucial role in inhibiting expression of the cartilage phenotype in inflammatory joint diseases.
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Protein Aliases: CMD1; CMPD1; gene 9; SRA1; SRY (sex determining region Y)-box9; SRY (sex-determining region Y)-box 9 protein; SRY box 9; SRY sex-determining region Y-box 9 protein; SRY-related HMG-box; SRY-related HMG-box, gene 9; Transcription factor SOX-9
Gene Aliases: CMD1; CMPD1; SOX9; SRA1; SRXX2; SRXY10
UniProt ID: (Human) P48436
Entrez Gene ID: (Human) 6662