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|Tested species reactivity||Not Applicable|
|Host / Isotype||E.coli|
|Storage buffer||0.05M tris HCl, pH 7.5, with 10mM L-glutathione|
|Storage Conditions||-20°C or -80°C if preferred|
|Tested Applications||Dilution *|
|Control (Ctrl)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This product is a GST-tagged recombinant protein corresponding to human SOX9, aa 1-150.
On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 43kDa (including GST).
SOX9 is a member of the family of SOX (Sry-type high mobility group box) gene that was first identified on the basis of region with high homology to that of Sry (Sex-determining region Y). It is a transcription factor with a high mobility group DNA-binding domain that is expressed in all pre-chondrocytic and chondrocytic cells during embryonic development in a pattern that closely parallels that of the gene for type II collagen. Phosphorylation of SOX9 by PKA increases its DNA-binding and transcriptional activity. SOX9 regulates expression of chondrocyte phenotype related genes. IL-1 and TNF-alpha can cause a marked and rapid decrease in the levels of SOX9 mRNA and/or protein in chondrocytes, thus strongly inhibiting the expression of genes for cartilage extracellular matrix proteins. The down-regulation of SOX9 may have a crucial role in inhibiting expression of the cartilage phenotype in inflammatory joint diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.