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SPAG4 Antibody (TA809875) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPAG4 (RC214367, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-SPAG4. Positive lysates LY418893 (100 µg) and LC418893 (20 µg) can be purchased separately from OriGene.
Product Details
TA809875
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI3E9
Immunogen
Human recombinant protein fragment corresponding to amino acids 191-279 of human SPAG4 produced in E.coli.
Conjugate
Unconjugated
Form
liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acrosomal protein ACR55; cancer/testis antigen 127; Outer dense fiber-associated protein SPAG4; Sad1 and UNC84 domain containing 4; sperm tail protein; Sperm-associated antigen 4 protein; SUN domain-containing protein 4
Gene Aliases: CT127; SPAG4; SUN4
UniProt ID: (Human) Q9NPE6
Entrez Gene ID: (Human) 6676
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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