|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||2 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Protein corresponding to amino acids 18–303 of human Osteonectin|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with mouse based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Osteonectin is a matrix-associated protein which is also known as Secreted Protein Acidic and Rich in Cysteine. It is a multifunctional protein with a high affinity for cations and hydroxyapatite that provides support to extracellular matrix and mediates the activities of a wide range of growth factors. In addition to this osteonectin is also known to play a vital role in tumor formation. Osteonectin is found to be present on the q arm of chromosome 5.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Basement-membrane protein 40; BM-40; ON; Osteonectin; Secreted protein acidic and rich in cysteine; secreted protein, acidic, cysteine-rich (osteonectin); SPARC
Gene Aliases: BM-40; OI17; ON; SPARC
UniProt ID: (Human) P09486
Entrez Gene ID: (Human) 6678
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