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Invitrogen
SPARC Monoclonal Antibody (5031)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
SPARC Antibody (MA1-43027) in WB
Western blot was performed using Anti-SPARC Monoclonal Antibody (5031) (Product # MA1-43027) and a 40 kDa band corresponding to SPARC was observed in BJ, U-2 OS, U-87 MG and SH-SY5Y which are reported to be positive and not in other cell lines like MCF7, HT-29 and Raji. Whole cell extracts (30 µg lysate) of BJ (Lane 1), U-2 OS (Lane 2), U-87 MG (Lane 3), SH-SY5Y (Lane 4), MCF7 (Lane 5), HT-29 (Lane 6) and Raji (Lane 7) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred... View More
Product Details
MA1-43027
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
5031
Immunogen
Human osteonectin.
Conjugate
Form
Liquid
Concentration
9.7 mg/mL
Purification
Ion-exchange chromatography, Ammonium sulfate precipitation
Storage buffer
50% water with 50% glycerol
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2195095
Product Specific Information
MA1-43027 detects SPARC in human, rat and bovine samples.
MA1-43027 has successfully been used in radioimmunoassays, Western blot, ELISA and immunohistochemistry applications.
MA1-43027 was derived from human platelet osteonectin.
Target Information
SPARC gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Basement membrane protein 40; Basement-membrane protein 40; BM-40; BM40; Cysteine rich protein; ON; Osteonectin; osteonectin precursor; Secreted acidic cystein rich glycoprotein; Secreted acidic cystein-rich glycoprotein (osteonectin); secreted acidic cysteine rich glycoprotein; Secreted protein acidic and rich in cysteine; Secreted protein acidic and rich in cysteine (SPARC); secreted protein, acidic, cysteine-rich (osteonectin); SPARC
Gene Aliases: BM-40; OI17; ON; SPARC
UniProt ID: (Bovine) P13213, (Human) P09486, (Rat) P16975
Entrez Gene ID: (Bovine) 282077, (Human) 6678, (Rat) 24791
ossification
negative regulation of endothelial cell proliferation
platelet degranulation
receptor-mediated endocytosis
signal transduction
heart development
response to gravity
response to lead ion
positive regulation of endothelial cell migration
negative regulation of angiogenesis
regulation of cell morphogenesis
extracellular matrix organization
lung development
response to lipopolysaccharide
response to L-ascorbic acid
response to cytokine
wound healing
response to peptide hormone
response to ethanol
response to cadmium ion
inner ear development
response to glucocorticoid
response to cAMP
response to calcium ion
bone development
cellular response to growth factor stimulus
regulation of cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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