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|Tested species reactivity||Human , Cattle|
|Published species reactivity||Rat , Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Bovine Osteonectin. Monoclonal antibody was obtained by fusing the mouse myeloma cell-line P3-X63-Ag8-U1 with spleen cells of BALB/c mouse after immunization with bovine Osteonectin (SPARC).|
|Purification||Ammonium sulfate precipitation, Ion-exchange chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1µg/ml|
|Western Blot (WB)||5-10µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is specific for the bone-derived and platelet-derived proteins. This antibody does not react with non-activated platelet, it reacts with thrombin-stimulated platelet (flow cytometric analysis).
Western blot can be performed using reducing or non-reducing conditions. This antibody was tested in Western blot using an EDTA-extracted fraction of bovine bone.
Reconstitute with 50µl distilled water for a final concentration of 2mg/ml. Reconstituted stock solution (2mg/mL) is stable at 4°C for 6 months, or at -20°C for 1 year. Store solution in aliquots, avoiding repeat freezing/thaw cycles.
Osteonectin is a matrix-associated protein which is also known as Secreted Protein Acidic and Rich in Cysteine. It is a multifunctional protein with a high affinity for cations and hydroxyapatite that provides support to extracellular matrix and mediates the activities of a wide range of growth factors. In addition to this osteonectin is also known to play a vital role in tumor formation. Osteonectin is found to be present on the q arm of chromosome 5.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Vasculogenic mimicry: a new prognostic sign of human osteosarcoma.
33-5500 was used in immunohistochemistry to evaluate the vasculogenic mimicry as a prognostic marker of human osteosarcoma
|Ren K,Yao N,Wang G,Tian L,Ma J,Shi X,Zhang L,Zhang J,Zhou X,Zhou G,Wu S,Sun X||Human pathology (45:2120)||2014|
Stromal responses among common carcinomas correlated with clinicopathologic features.
33-5500 was used in immunohistochemistry to test if the desmoid-type fibromatosis signature is specific to breast cancer or if it is found in multiple types of cancer.
|Chen JL,Espinosa I,Lin AY,Liao OY,van de Rijn M,West RB||Clinical cancer research : an official journal of the American Association for Cancer Research (19:5127)||2013|
Gene expression in the normal adult human kidney assessed by complementary DNA microarray.
||Higgins JP,Wang L,Kambham N,Montgomery K,Mason V,Vogelmann SU,Lemley KV,Brown PO,Brooks JD,van de Rijn M||Molecular biology of the cell (15:649)||2004|
Effects of bisphosphonates on sutural bone formation and relapse: A histologic and immunohistochemical study.
||Oztürk F,Babacan H,Inan S,Gümüş C||American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics (140:e31)||2011|
SPARC/osteonectin is a frequent target for aberrant methylation in pancreatic adenocarcinoma and a mediator of tumor-stromal interactions.
||Sato N,Fukushima N,Maehara N,Matsubayashi H,Koopmann J,Su GH,Hruban RH,Goggins M||Oncogene (22:5021)||2003|
Gene expression profiling of normal human pulmonary fibroblasts following coculture with non-small-cell lung cancer cells reveals alterations related to matrix degradation, angiogenesis, cell growth and survival.
||Fromigué O,Louis K,Dayem M,Milanini J,Pages G,Tartare-Deckert S,Ponzio G,Hofman P,Barbry P,Auberger P,Mari B||Oncogene (22:8487)||2003|
BM-40, SPARC, basement-membrane protein 40, cysteine-rich protein, osteonectin, secreted protein acidic and rich in cysteine, ON