OriGene

SPG7 Monoclonal Antibody (OTI1C12), TrueMAB™

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SPG7 Antibody in Immunocytochemistry (ICC/IF) — Anti-SPG7 mouse monoclonal antibody (TA504420) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7(RC206340).

SPG7 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (RC206340, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-SPG7. Positive lysates LY401085 (100 µg) and LC401085 (20 µg) can be purchased separately from OriGene.

SPG7 Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC206340 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SPG7 antibody (TA504420), and then analyzed by flow cytometry.

Product Details

TA504420

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunocytochemistry (ICC/IF)	1:50-1:100	-
Flow Cytometry (Flow)	1:100	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	OTI1C12
Immunogen	Human recombinant protein fragment corresponding to amino acids 300-573 of human SPG7 prodduced in E.coli. View antigen
Conjugate	Unconjugated
Form	liquid
Concentration	0.86 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Target Information

This gene encodes a mitochondrial metalloprotease protein that is a member of the AAA family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Mutations in this gene cause autosomal recessive spastic paraplegia 7. Two transcript variants encoding distinct isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cell adhesion regulator; Cell matrix adhesion regulator; Paraplegin; paraplegin, isoform 1; spastic paraplegia 7 (pure and complicated autosomal recessive); Spastic paraplegia 7 protein

Gene Aliases: AI452278; AU015315; CAR; CMAR; PGN; SPG5C; SPG7

UniProt ID: (Human) Q9UQ90, (Mouse) Q3ULF4

Entrez Gene ID: (Human) 6687, (Mouse) 234847

Molecular Function: metalloprotease