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Product Details
MA5-32709
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JA31-14
Immunogen
Synthetic peptide within Human SPHK1 aa 25-74
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809986
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
SPHK1, along with SPHK2, catalyzes the phosphorylation of sphingosine producing the signaling molecule sphingosine-1-phosphate (S1P). S1P signals through G-protein coupled receptors and controls diverse cellular processes. Inhibitors of SPHKs are viewed as potential therapeutics for the treatment of cancer. SPHK1 catalyzes the phosphorylation of sphingosine to sphingosine 1 phosphate (S1P), an important signaling molecule with intra- and extracellular functions. Inside the cell S1P acts as a signaling molecule like other sphingolipid metabolites like ceramide and sphingosine. S1P has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acetyltransferase SPHK1; RP23-193A16.4; SK 1; SKase; Sphingosine kinase 1; SPK 1
Gene Aliases: SK1; SPHK; SPHK1; SPK
UniProt ID: (Human) Q96GK1
Entrez Gene ID: (Human) 8877
blood vessel development
positive regulation of neurotransmitter secretion
sphingosine-1-phosphate signaling pathway
protein folding
sphingosine metabolic process
inflammatory response
signal transduction
brain development
female pregnancy
cellular response to starvation
positive regulation of peptidyl-threonine phosphorylation
regulation of tumor necrosis factor-mediated signaling pathway
positive regulation of neuron projection development
response to amine
cyclooxygenase pathway
calcium-mediated signaling
sphingolipid biosynthetic process
positive regulation of cell growth
positive regulation of cell migration
positive regulation of protein ubiquitination
response to magnesium ion
response to progesterone
regulation of interleukin-1 beta production
response to ATP
intracellular signal transduction
positive regulation of NF-kappaB import into nucleus
negative regulation of apoptotic process
positive regulation of angiogenesis
positive regulation of mitotic cell cycle
positive regulation of smooth muscle contraction
sphingosine biosynthetic process
sphingoid catabolic process
lipid phosphorylation
positive regulation of fibroblast proliferation
positive regulation of NF-kappaB transcription factor activity
cellular response to hydrogen peroxide
response to interleukin-1
cellular response to growth factor stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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