|Tested species reactivity||Human|
|Published species reactivity||Rabbit, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human SPP1 (amino acids: 167-314) expressed in E. Coli.|
|Storage buffer||PBS with 0.5% proprietary stabilizer|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1:200 - 1:1000|
|Immunofluorescence (IF)||1:200 - 1:1000|
|Immunohistochemistry (IHC)||1:200 - 1:1000|
|Western Blot (WB)||1:500 - 1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17180 targets SPP1 in ICC, IHC, IF and WB applications and shows reactivity with Human samples.
The MA5-17180 immunogen is purified recombinant fragment of human SPP1 (amino acids: 167-314) expressed in E. Coli.
MA5-17180 detects SPP1 which has a predicted molecular weight of approximately 35.4kDa.
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Regeneration of a goat femoral head using a tissue-specific, biphasic scaffold fabricated with CAD/CAM technology.
MA5-17180 was used in immunohistochemistry to study the use of CAD/CAM technology to aid the construction of biphasic scaffolds for the tissue engineering repair of the femoral head in a goat model
|Ding C,Qiao Z,Jiang W,Li H,Wei J,Zhou G,Dai K||Biomaterials (34:6706)||2013|
Restoration of critical-size defects in the rabbit mandible using porous nanohydroxyapatite-polyamide scaffolds.
MA5-17180 was used in immunocytochemistry to study the use of porous nanohydroxyapatite-polyamide-based scaffolds to aid the repair of rabbit mandible bone defects
|Guo J,Meng Z,Chen G,Xie D,Chen Y,Wang H,Tang W,Liu L,Jing W,Long J,Guo W,Tian W||Tissue engineering. Part A (18:1239)||2012|