Invitrogen

SR Monoclonal Antibody (16H3 (16H3E8))

3 Published Figures

19 References

View all (2) SR antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (3)

FIGURE: 1 / 4

SR Antibody (33-9300) in WB

Western blot analysis of SR proteins expression in HeLa cell lysate using SR Protein Monoclonal Antibody, Mouse

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Product Details

33-9300

Applications

Tested Dilution

Publications

Western Blot (WB)

3-5 µg/mL

View 12 publications 12 publications

Immunocytochemistry (ICC/IF)

0.5-2 µg/mL

View 2 publications 2 publications

ELISA (ELISA)

0.1-1 µg/mL

Immunoprecipitation (IP)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Fruit fly, Human, Mouse, Rabbit, Rat, Xenopus

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

16H3 (16H3E8)

Immunogen

Purified, de-phosphorylated bovine Srp55 and Drosophila p55-GST. The 16H3 epitope has been mapped to a 40-amino acid polypeptide composed almost exclusively of arginine alternating with glutamate and aspartate

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533149

Product Specific Information

This monoclonal antibody can be used to detect a sub-set of the non-snRNP splicing factors termed SR proteins. The antibody detects SRp75, SRp55, SRp40, and SRp20 but not SRp30a or b (ASF/SF2, SC-35) proteins. 16H3 also recognizes approximately 20 distinct nuclear proteins including the U1 70K component of the U1 snRNP and both subunits of U2AF. The epitope detected by the 16H3 monoclonal antibody has been termed the “alternating arginine domain” and is composed almost exclusively of argnine alternating with glutamate and aspartate. 16H3 targets are predominantly nuclear, nonnucleolar, and are localized to active sites of polymerase II transcription.

Target Information

SR proteins are a highly conserved family of arginine/serine-rich, spliceosome-associated phosphoproteins essential for metazoan pre-mRNA splicing. SR proteins act early in splicing by promoting splice site recognition and spliceosome assembly. SR proteins also play a regulatory role because they can determine alternative splice site usage in vivo and in vitro. SR proteins appear to be recruited from nuclear “speckles”, in which they are concentrated to sites of transcription in order to spatially coordinate transcription and pre-mRNA splicing within the cell nucleus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Antisense Oligonucleotide Rescue of Deep-Intronic Variants Activating Pseudoexons in the 6-Pyruvoyl-Tetrahydropterin Synthase Gene.

Nucleic acid therapeutics

Martínez-Pizarro A,Leal F,Holm LL,Doktor TK,Petersen USS,Bueno M,Thöny B,Pérez B,Andresen BS,Desviat LR

33-9300 was used in Western Blotting to report two new 6-pyruvoyl-tetrahydropterin synthase splicing variants identified through genomic sequencing and transcript analysis in a patient with tetrahydrobiopterin deficiency, presenting with hyperphenylalaninemia and monoamine neurotransmitter deficiency.

Sat Oct 01 00:00:00 EDT 2022

Alteration of SC35 localization by transfection reagents.

Biochimica et biophysica acta. Molecular cell research

Damodaran AP,Courthéoux T,Watrin E,Prigent C

Wed Apr 01 00:00:00 EDT 2020

Intronic PAH gene mutations cause a splicing defect by a novel mechanism involving U1snRNP binding downstream of the 5' splice site.

PLoS genetics

Martínez-Pizarro A,Dembic M,Pérez B,Andresen BS,Desviat LR

33-9300 was used in Western Blotting to indicate that U1snRNP binding downstream of the natural 5' splice site determines efficient exon 11 splicing.

Sun Apr 01 00:00:00 EDT 2018

PRMT1-mediated arginine methylation controls ATXN2L localization.

Experimental cell research

Kaehler C,Guenther A,Uhlich A,Krobitsch S

33-9300 was used in immunocytochemistry to examine the methylation state of ATXN2L and its contribution to ATXN2L localization

Fri May 15 00:00:00 EDT 2015

Human TREX component Thoc5 affects alternative polyadenylation site choice by recruiting mammalian cleavage factor I.

Nucleic acids research

Katahira J,Okuzaki D,Inoue H,Yoneda Y,Maehara K,Ohkawa Y

Thu Aug 01 00:00:00 EDT 2013

Human TREX component Thoc5 affects alternative polyadenylation site choice by recruiting mammalian cleavage factor I.

Nucleic acids research

Katahira J,Okuzaki D,Inoue H,Yoneda Y,Maehara K,Ohkawa Y

Thu Aug 01 00:00:00 EDT 2013

Paraquat modulates alternative pre-mRNA splicing by modifying the intracellular distribution of SRPK2.

PloS one

Vivarelli S,Lenzken SC,Ruepp MD,Ranzini F,Maffioletti A,Alvarez R,Mühlemann O,Barabino SM

33-9300 was used in western blot to explore the mechanism underlying paraquat-induced pre-mRNA splicing alterations.

Thu Nov 14 00:00:00 EST 2013

Paraquat modulates alternative pre-mRNA splicing by modifying the intracellular distribution of SRPK2.

PloS one

Vivarelli S,Lenzken SC,Ruepp MD,Ranzini F,Maffioletti A,Alvarez R,Mühlemann O,Barabino SM

33-9300 was used in western blot to explore the mechanism underlying paraquat-induced pre-mRNA splicing alterations.

Thu Nov 14 00:00:00 EST 2013

Serine arginine splicing factor 3 is involved in enhanced splicing of glucose-6-phosphate dehydrogenase RNA in response to nutrients and hormones in liver.

The Journal of biological chemistry

Walsh CM,Suchanek AL,Cyphert TJ,Kohan AB,Szeszel-Fedorowicz W,Salati LM

33-9300 was used in western blot to report that nutrients and hormones control the activity of serine-arginine-rich proteins which regulate the splicing of G6PD mRNA.

Fri Jan 25 00:00:00 EST 2013

Engineering DYRK1A overdosage yields Down syndrome-characteristic cortical splicing aberrations.

Neurobiology of disease

Toiber D,Azkona G,Ben-Ari S,Torán N,Soreq H,Dierssen M

33-9300 was used in western blot to assess the splicing machinery in Down syndrome brains

Fri Oct 01 00:00:00 EDT 2010

Engineering DYRK1A overdosage yields Down syndrome-characteristic cortical splicing aberrations.

Neurobiology of disease

Toiber D,Azkona G,Ben-Ari S,Torán N,Soreq H,Dierssen M

33-9300 was used in western blot to assess the splicing machinery in Down syndrome brains

Fri Oct 01 00:00:00 EDT 2010

Antagonistic roles of four SR proteins in the biosynthesis of alternatively spliced tissue factor transcripts in monocytic cells.

Journal of leukocyte biology

Chandradas S,Deikus G,Tardos JG,Bogdanov VY

33-9300 was used in western blot to identify factors that modulate TF pre-mRNA splicing

Fri Jan 01 00:00:00 EST 2010

5-(N-ethyl-N-isopropyl)-amiloride enhances SMN2 exon 7 inclusion and protein expression in spinal muscular atrophy cells.

Annals of neurology

Yuo CY,Lin HH,Chang YS,Yang WK,Chang JG

33-9300 was used in western blot to investigate if inhibitors of the Na+/H+ exchanger modulate the exon 7 splicing of survival motor neuron 2 mRNA in spinal muscular atrophy patients

Tue Jan 01 00:00:00 EST 2008

Negative and positive mRNA splicing elements act competitively to regulate human immunodeficiency virus type 1 vif gene expression.

Journal of virology

Exline CM,Feng Z,Stoltzfus CM

33-9300 was used in immunoprecipitation to determine whether mutations within 5' splice site downstream of exon2 result in reduced or increased HIV-1 Vif expression

Tue Apr 01 00:00:00 EDT 2008

Extracellular pH change modulates the exon 7 splicing in SMN2 mRNA.

Molecular and cellular neurosciences

Chen YC,Yuo CY,Yang WK,Jong YJ,Lin HH,Chang YS,Chang JG

33-9300 was used in western blot to examine the effect of extracellular pH on SMN2 exon 7 splicing in spinal muscular atrophy cells

Wed Oct 01 00:00:00 EDT 2008

The effect of hydroxyurea in spinal muscular atrophy cells and patients.

Journal of the neurological sciences

Liang WC,Yuo CY,Chang JG,Chen YC,Chang YF,Wang HY,Ju YH,Chiou SS,Jong YJ

33-9300 was used in western blot to determine the effect of hydroxyurea in spinal muscular atrophy cells and patients

Thu May 15 00:00:00 EDT 2008

Combination of Clk family kinase and SRp75 modulates alternative splicing of Adenovirus E1A.

Genes to cells : devoted to molecular & cellular mechanisms

Yomoda J,Muraki M,Kataoka N,Hosoya T,Suzuki M,Hagiwara M,Kimura H

33-9300 was used in western blot to assess how SR kinases play a role in alternative splicing through dynamic balance of phosphorylation

Sat Mar 01 00:00:00 EST 2008

The switch in alternative splicing of cyclic AMP-response element modulator protein CREM{tau}2{alpha} (activator) to CREM{alpha} (repressor) in human myometrial cells is mediated by SRp40.

The Journal of biological chemistry

Tyson-Capper AJ,Bailey J,Krainer AR,Robson SC,Europe-Finner GN

Fri Oct 14 00:00:00 EDT 2005

AEC-associated p63 mutations lead to alternative splicing/protein stabilization of p63 and modulation of Notch signaling.

Cell cycle (Georgetown, Tex.)

Huang YP,Kim Y,Li Z,Fomenkov T,Fomenkov A,Ratovitski EA

33-9300 was used in western blot to determine the molecular mechanisms associated with ankyloblepharon, ectodermal dysplasia, and clefting

Sat Oct 01 00:00:00 EDT 2005

Treatment of spinal muscular atrophy by sodium butyrate.

Proceedings of the National Academy of Sciences of the United States of America

Chang JG,Hsieh-Li HM,Jong YJ,Wang NM,Tsai CH,Li H

Tue Aug 14 00:00:00 EDT 2001

Treatment of spinal muscular atrophy by sodium butyrate.

Proceedings of the National Academy of Sciences of the United States of America

Chang JG,Hsieh-Li HM,Jong YJ,Wang NM,Tsai CH,Li H

Tue Aug 14 00:00:00 EDT 2001

Treatment of spinal muscular atrophy by sodium butyrate.

Proceedings of the National Academy of Sciences of the United States of America

Chang JG,Hsieh-Li HM,Jong YJ,Wang NM,Tsai CH,Li H

Tue Aug 14 00:00:00 EDT 2001

Bioinformatics

Protein Aliases: Delayed-early protein HRS; epididymis secretory protein Li 91; Insulin-induced growth response protein CL-4; pre-mRNA splicing factor SRp20; Pre-mRNA-splicing factor SRP20; Pre-mRNA-splicing factor SRP40; Pre-mRNA-splicing factor SRP55; Pre-mRNA-splicing factor SRP75; Protein X16; Serine/arginine-rich splicing factor 3; Serine/arginine-rich splicing factor 4; Serine/arginine-rich splicing factor 5; Serine/arginine-rich splicing factor 6; splicing factor arginine/serine-rich 5 (SRp40 HRS); splicing factor SRp55; splicing factor SRp55-1; Splicing factor, arginine/serine-rich 3; splicing factor, arginine/serine-rich 3 (SRp20); Splicing factor, arginine/serine-rich 4; splicing factor, arginine/serine-rich 4 (SRp75); Splicing factor, arginine/serine-rich 5; splicing factor, arginine/serine-rich 5 (SRp40, HRS); Splicing factor, arginine/serine-rich 6; splicing factor, arginine/serine-rich, 20-kD; splicing factor, arginine/serine-rich, 55 kDa; SR splicing factor 4; SR splicing factor 5; SR splicing factor 6; SRP001LB

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Gene Aliases: 1210001E11Rik; 5730499P16Rik; AI314910; AL024116; AW146126; AW550192; B52; Cl-4; HEL-S-91; HRS; MNCb-2616; RGD1561347; SFRS3; SFRS4; SFRS5; SFRS6; SRP20; SRP40; SRP55; SRP55-1; SRP75; SRSF3; SRSF4; SRSF5; SRSF6; X16

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UniProt ID: (Human) P84103, (Bovine) Q3SZR8, (Human) Q08170, (Human) Q13243, (Bovine) Q3B7L6, (Human) Q13247, (Mouse) P84104, (Mouse) O35326, (Rat) Q09167, (Rat) G3V6S8, (Mouse) Q3TWW8

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Function(s)

nucleotide binding RNA binding protein binding phospholipase binding poly(A) RNA binding sequence-specific mRNA binding RS domain binding pre-mRNA binding nucleic acid binding RNA splicing factor

Process(es)

mRNA splicing, via spliceosome termination of RNA polymerase II transcription RNA export from nucleus mRNA export from nucleus mRNA 3'-end processing regulation of mRNA splicing, via spliceosome cellular response to leukemia inhibitory factor RNA splicing, via transesterification reactions mRNA processing RNA splicing negative regulation of mRNA splicing, via spliceosome mRNA splice site selection response to wounding cellular response to insulin stimulus regulation of cell cycle liver regeneration alternative mRNA splicing, via spliceosome regulation of alternative mRNA splicing, via spliceosome regulation of keratinocyte proliferation negative regulation of keratinocyte differentiation regulation of wound healing positive regulation of epithelial cell proliferation involved in lung morphogenesis negative regulation of cell death transport insulin receptor signaling pathway mRNA transport hematopoietic progenitor cell differentiation transcription, DNA-templated regulation of transcription, DNA-templated

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SR Monoclonal Antibody (16H3 (16H3E8))

SR Antibody (33-9300) in WB

Product Details

33-9300

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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