|Tested species reactivity||Human, Xenopus|
|Published species reactivity||Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Mass-isolated nuclei from Xenopus Laevis oocytes.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
SR proteins appear to be recruited from nuclear "speckles", in which they are concentrated, to sites of transcription in order to spatially coordinate transcription and pre-mRNA splicing within the cell nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Balanced splicing at the Tat-specific HIV-1 3'ss A3 is critical for HIV-1 replication.
33-9400 was used in western blot to investigate viral tat-mRNA splicing.
|Erkelenz S,Hillebrand F,Widera M,Theiss S,Fayyaz A,Degrandi D,Pfeffer K,Schaal H||Retrovirology (12:null)||2015|
Serine arginine splicing factor 3 is involved in enhanced splicing of glucose-6-phosphate dehydrogenase RNA in response to nutrients and hormones in liver.
33-9400 was used in ChIP assay and western blot to report that nutrients and hormones control the activity of serine-arginine-rich proteins which regulate the splicing of G6PD mRNA.
|Walsh CM,Suchanek AL,Cyphert TJ,Kohan AB,Szeszel-Fedorowicz W,Salati LM||The Journal of biological chemistry (288:2816)||2013|
Engineering DYRK1A overdosage yields Down syndrome-characteristic cortical splicing aberrations.
33-9400 was used in immunohistochemistry and western blot to assess the splicing machinery in Down syndrome brains
|Toiber D,Azkona G,Ben-Ari S,Torán N,Soreq H,Dierssen M||Neurobiology of disease (40:348)||2010|
Digoxin suppresses HIV-1 replication by altering viral RNA processing.
33-9400 was used in western blot to examine the effects of RNA splicing modulators on viral RNA metabolism
|Wong RW,Balachandran A,Ostrowski MA,Cochrane A||PLoS pathogens (9:null)||2013|
|Human||1:1000||The cardiotonic steroid digitoxin regulates alternative splicing through depletion of the splicing factors SRSF3 and TRA2B.||Anderson ES,Lin CH,Xiao X,Stoilov P,Burge CB,Black DL||RNA (New York, N.Y.) (18:1041)||2012|
|Not Applicable||Not Cited||
Changes in brain MicroRNAs contribute to cholinergic stress reactions.
33-9400 was used in western blot to study how stress-regulated miRs regulate alternative splicing
|Meerson A,Cacheaux L,Goosens KA,Sapolsky RM,Soreq H,Kaufer D||Journal of molecular neuroscience : MN (40:47)||2010|
|Mouse||Not Cited||RNA folding affects the recruitment of SR proteins by mouse and human polypurinic enhancer elements in the fibronectin EDA exon.||Buratti E,Muro AF,Giombi M,Gherbassi D,Iaconcig A,Baralle FE||Molecular and cellular biology (24:1387)||2004|
|Rat||Not Cited||RNA splicing capability of live neuronal dendrites.||Glanzer J,Miyashiro KY,Sul JY,Barrett L,Belt B,Haydon P,Eberwine J||Proceedings of the National Academy of Sciences of the United States of America (102:16859)||2005|
||RNA folding affects the recruitment of SR proteins by mouse and human polypurinic enhancer elements in the fibronectin EDA exon.||Buratti E,Muro AF,Giombi M,Gherbassi D,Iaconcig A,Baralle FE||Molecular and cellular biology (24:1387)||2004|