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Western blot analysis was performed on nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), HCT 116 (Lane 2), HT-29 (Lane 3), A549 (Lane 4) and Hep G2 (Lane 5). The blot was probed with Anti-SRP20 Mouse Monoclonal Antibody (Product # 33-4200, 2 µg/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjµgate (Product # A28177, 0.4 µg/ml, 1:2500 dilution). A ~25 kDa band corresponding to SRP20 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human|
|Published species reactivity||Human, Xenopus|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||A synthetic peptide corresponding to amino acids 84-104 of the human SRp20 protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
SR proteins appear to be recruited from nuclear "speckles", in which they are concentrated, to sites of transcription in order to spatially coordinate transcription and pre-mRNA splicing within the cell nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Characterization of novel inhibitors of HIV-1 replication that function via alteration of viral RNA processing and rev function.
33-4200 was used in immunocytochemistry to elucidate the mechanisms of action of two small molecule modulators of HIV- RNA processing.
|Wong RW,Balachandran A,Haaland M,Stoilov P,Cochrane A||Nucleic acids research (41:9471)||2013|
|Human||1:3500||The cardiotonic steroid digitoxin regulates alternative splicing through depletion of the splicing factors SRSF3 and TRA2B.||Anderson ES,Lin CH,Xiao X,Stoilov P,Burge CB,Black DL||RNA (New York, N.Y.) (18:1041)||2012|
|Human||1:4000||Regulation of alternative splicing by SRrp86 and its interacting proteins.||Li J,Hawkins IC,Harvey CD,Jennings JL,Link AJ,Patton JG||Molecular and cellular biology (23:7437)||2003|
|SR splicing factors serve as adapter proteins for TAP-dependent mRNA export.||Huang Y,Gattoni R,Stévenin J,Steitz JA||Molecular cell (11:837)||2003|
|Human||Not Cited||ICP27 interacts with SRPK1 to mediate HSV splicing inhibition by altering SR protein phosphorylation.||Sciabica KS,Dai QJ,Sandri-Goldin RM||The EMBO journal (22:1608)||2003|
arginine/serine-rich 3; pre-mRNA splicing factor SRp20; pre-mRNA-splicing factor SRP20; SFRS3; Splicing factor; splicing factor, arginine/serine-rich 3; splicing factor, arginine/serine-rich, 20-kD; SRP20
SFRS3; SRP20; SRSF3