|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||KLH conjugated peptide corresponding to amino acid residues 519-536 of human c-Src.|
|Storage buffer||PBS, pH 7.2, with 1% BSA|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||1.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. Transcript Variant: This variant (1) represents the longer transcript. Both isoforms 1 and 2 encode the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Deregulated Expression of SRC, LYN and CKB Kinases by DNA Methylation and Its Potential Role in Gastric Cancer Invasiveness and Metastasis.
AHO0051 was used in immunohistochemistry - paraffin section and western blot to assess SRC, LYN, and CKB expression, as well as their promoter methylation, in gastric cancer
|Mello AA,Leal MF,Rey JA,Pinto GR,Lamarão LM,Montenegro RC,Alves AP,Assumpção PP,Borges Bdo N,Smith MC,Burbano RR||PloS one (10:null)||2015|
Post-ovulatory aging of oocytes disrupts kinase signaling pathways and lysosome biogenesis.
AHO0051 was used in western blot to assess broad patterns of signaling pathway activity during in vitro oocyte aging.
|McGinnis LK,Pelech S,Kinsey WH||Molecular reproduction and development (81:928)||2014|
Extracellular domain dependence of PTPalpha transforming activity.
AHO0051 was used in western blot to investigate the functions of PTPalpha isoforms
|Tremper-Wells B,Resnick RJ,Zheng X,Holsinger LJ,Shalloway D||Genes to cells : devoted to molecular and cellular mechanisms (15:711)||2010|
UNC119a bridges the transmission of Fyn signals to Rab11, leading to the completion of cytokinesis.
AHO0051 was used in western blot to investigate UNC9a interactions during cytokinesis.
|Lee Y,Chung S,Baek IK,Lee TH,Paik SY,Lee J||Cell cycle (Georgetown, Tex.) (12:1303)||2013|
|Not Applicable||Not Cited||
Regulation of c-Src nonreceptor tyrosine kinase activity by bengamide A through inhibition of methionine aminopeptidases.
AHO0051 was used in western blot to examine the effects of N-myristoylation on c-Src
|Hu X,Dang Y,Tenney K,Crews P,Tsai CW,Sixt KM,Cole PA,Liu JO||Chemistry and biology (14:764)||2007|
|Mouse||Not Cited||Analyzing FAK and Pyk2 in early integrin signaling events.||Bernard-Trifilo JA,Lim ST,Hou S,Schlaepfer DD,Ilic D||Current protocols in cell biology (Chapter 14:null)||2006|
|Not Applicable||2.5 µg/ml||
Fertilization triggers localized activation of Src-family protein kinases in the zebrafish egg.
AHO0051 was used in immunohistochemistry - frozen section to determine the subcellular distribution of activated Src-family kinases in the fertilized zebrafish egg
|Sharma D,Kinsey WH||Developmental biology (295:604)||2006|
||Analyzing FAK and Pyk2 in early integrin signaling events.||Bernard-Trifilo JA,Lim ST,Hou S,Schlaepfer DD,Ilic D||Current protocols in cell biology (Chapter 14:null)||2006|
|Mouse||Not Cited||Impairment of decidualization in SRC-deficient mice.||Shimizu A,Maruyama T,Tamaki K,Uchida H,Asada H,Yoshimura Y||Biology of reproduction (73:1219)||2005|