|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human SRC expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/200 - 1/400|
|Immunohistochemistry (IHC)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA5-15924 targets SRC in FACS, IHC, and WB applications and shows reactivity with Human samples.
The MA5-15924 immunogen is purified recombinant fragment of human SRC expressed in E. Coli. .
MA5-15924 detects SRC which has a predicted molecular weight of approximately 60kDa.
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Contrasting effects of prenyltransferase inhibitors on estrogen-dependent cell cycle progression and estrogen receptor-mediated transcriptional activity in MCF-7 cells.
MA5-15924 was used in western blot to study the effect of prenyltransferase inhibitors on estrogen-dependent cell cycle progression and on transcriptional activity mediated by estrogen receptor-alpha
|Doisneau-Sixou SF,Cestac P,Chouini S,Carroll JS,Hamilton AD,Sebti SM,Poirot M,Balaguer P,Faye JC,Sutherland RL,Favre G||Endocrinology (144:989)||2003|