|Tested species reactivity||Rat|
|Host / Isotype||Sheep / IgG|
|Immunogen||Recombinant protein corresponding to residues 620-1596 of SSeCKS.|
|Purification||Ammonium sulfate precipitation|
|Contains||0.08% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is rat aortic smooth muscle lysate.
The Src family of protein tyrosine kinases are non-receptor kinases whose role is important in the regulation of growth and differentiation of eukaryotic cells (1,2). Activity of Src is regulated phosphorylation of two tyrosine residues (Tyr 416 and Tyr527). Phosphorylation of these two sites has opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates enzyme activity. It has been shown that the phosphorylation of Tyr527 decreases enzyme activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.