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Western Blot. Analysis Extracts of CEFs untransfected (-) or transfected (+) with wild-type human Src were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to nitrocellulose. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4°C, then incubated with 0.5 µg/ml Src pan antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, the membrane was incubated with goat F(ab"e;)2 anti-rabbit IgG alkaline phosphatase (Cat. no. ALI4405) and signals were detected using the Tropix WesternStar™ method. The data show the total level of Src present in each condition
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Dog, Rat, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized peptide derived from the amino acid region 31-49 of human Src protein. The sequence is conserved in mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. Transcript Variant: This variant (1) represents the longer transcript. Both isoforms 1 and 2 encode the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Ouabain induces endocytosis and degradation of tight junction proteins through ERK1/2-dependent pathways.
44-656G was used in western blot to investigate the effect of ouabain on endocytosis and degradation of tight junction proteins.
|Rincon-Heredia R,Flores-Benitez D,Flores-Maldonado C,Bonilla-Delgado J,García-Hernández V,Verdejo-Torres O,Castillo AM,Larré I,Poot-Hernández AC,Franco M,Gariglio P,Reyes JL,Contreras RG||Experimental cell research (320:108)||2014|
|Human||1:500||Different changes in protein and phosphoprotein levels result from serum starvation of high-grade glioma and adenocarcinoma cell lines.||Levin VA,Panchabhai SC,Shen L,Kornblau SM,Qiu Y,Baggerly KA||Journal of proteome research (9:179)||2010|
|Mouse||Not Cited||Analyzing FAK and Pyk2 in early integrin signaling events.||Bernard-Trifilo JA,Lim ST,Hou S,Schlaepfer DD,Ilic D||Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.] (Chapter 14:null)||2006|
|Mouse||Not Cited||Different modes and qualities of tyrosine phosphorylation of Fak and Pyk2 during epithelial-mesenchymal transdifferentiation and cell migration: analysis of specific phosphorylation events using site-directed antibodies.||Nakamura K,Yano H,Schaefer E,Sabe H||Oncogene (20:2626)||2001|
Biophysical stimulation induces demyelination via an integrin-dependent mechanism.
44-656G was used in immunohistochemistry to identify the physical stimuli mediating demyelination and test if integrins plays a role in the demyelinating response.
|Lin MY,Frieboes LS,Forootan M,Palispis WA,Mozaffar T,Jafari M,Steward O,Gall CM,Gupta R||Annals of neurology (72:112)||2012|
||Analyzing FAK and Pyk2 in early integrin signaling events.||Bernard-Trifilo JA,Lim ST,Hou S,Schlaepfer DD,Ilic D||Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.] (Chapter 14:null)||2006|
||Different modes and qualities of tyrosine phosphorylation of Fak and Pyk2 during epithelial-mesenchymal transdifferentiation and cell migration: analysis of specific phosphorylation events using site-directed antibodies.||Nakamura K,Yano H,Schaefer E,Sabe H||Oncogene (20:2626)||2001|
c-Src; EC 188.8.131.52; neuronal proto-oncogene tyrosine-protein kinase Src; p60-Src; proto-oncogene c-Src; protooncogene SRC, Rous sarcoma; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
ASV; AW259666; c-SRC; p60-Src; pp60c-src; SRC; SRC1; THC6