Bethyl Laboratories

SRC1 Polyclonal Antibody

View all (10) SRC1 antibodies

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SRC1 Antibody in Western Blot (WB) — Detection of human and mouse SRC1 by western blot. Samples: Whole cell lysate from 293T (50, 15, 5 µg), and (50 µg) from HeLa, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SRC1 antibody A300-343A-M (lot A300-343A-M-2) used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.

Product Details

Tested Applications

Dilution

Immunohistochemistry (IHC)

1:50-1:200

Western Blot (WB)

1:1,000

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Between 600 and 650

Conjugate

Unconjugated

Form

liquid

Purification

Antigen affinity chromatography

Storage buffer

TBS with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Target Information

Clone 1135 recognizes SRC-1, steroid-receptor coactivator-1. SRC-1 is a member of a gene family that functions in the full transcriptional activation of the steroid hormone receptor superfamily and contains intrinsic histone acetyltransferase activity. SRC-1 has a molecular weight of 160 kDa and has a basic helix-loop-helix (bHLH)-PAS domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ASV; bHLHe74; c-SRC; Class E basic helix-loop-helix protein 74; Hin-2 protein; mNRC-1; NCoA-1; Nuclear receptor coactivator 1; Nuclear receptor coactivator protein 1; PAX3/NCOA1 fusion protein; Protein Hin-2; Renal carcinoma antigen NY-REN-52; RIP160; SRC-1; SRC-a/NCoA-1; SRC1; Steroid receptor coactivator 1; steroid receptor coactivator-1

Gene Aliases: bHLHe42; BHLHE74; F-SRC-1; KAT13A; NCOA1; RIP160; SRC-1; SRC1

UniProt ID: (Human) Q15788, (Mouse) P70365

Entrez Gene ID: (Human) 8648, (Mouse) 17977

Molecular Function: acetyltransferase transcription factor transferase

positive regulation of transcription from RNA polymerase II promoter by galactose regulation of thyroid hormone mediated signaling pathway transcription, DNA-templated lactation male gonad development bile acid and bile salt transport cerebellum development hippocampus development hypothalamus development cerebral cortex development androgen receptor signaling pathway intracellular receptor signaling pathway response to estradiol response to retinoic acid response to progesterone cellular response to hormone stimulus positive regulation of apoptotic process histone H4 acetylation cellular lipid metabolic process estrous cycle positive regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of female receptivity positive regulation of transcription from RNA polymerase II promoter male mating behavior labyrinthine layer morphogenesis cellular response to Thyroglobulin triiodothyronine regulation of cellular response to drug regulation of transcription, DNA-templated signal transduction regulation of nucleic acid-templated transcription regulation of RNA biosynthetic process

Process(es)

RNA polymerase II regulatory region DNA binding RNA polymerase II transcription coactivator activity chromatin binding transcription coactivator activity histone acetyltransferase activity protein binding ligand-dependent nuclear receptor binding enzyme binding estrogen receptor binding ligand-dependent nuclear receptor transcription coactivator activity protein complex binding progesterone receptor binding nuclear hormone receptor binding retinoid X receptor binding protein dimerization activity protein N-terminus binding androgen receptor binding DNA binding signal transducer activity transcription factor binding transferase activity transferase activity, transferring acyl groups retinoic acid receptor binding