MA1-840 detects steroid receptor coactivator-1 (SRC-1) from mouse and primate tissues and cells as well as recombinant human SRC-1 protein.
MA1-840 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot MA1-840 detects an ~165 kDa protein representing SRC-1 in COS cell extract. Also by Western blot, MA1-840 detects an unidentified protein at ~30-40 kDa. This antibody does not react with NIH-3T3 cells in Western blot.
The MA1-840 immunogen is a synthetic peptide corresponding to residues 477-947 of human SRC-1. The epitope for MA1-840 has been mapped to the extreme C-terminal end of this fragment.
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. One such coactivator is Steroid Receptor Coactivator-1 (SRC-1).
SRC-1 has been shown to interact with and stimulate the transcriptional activity of estrogen, progesterone, retinoic acid, thyroid and glucocorticoid receptors, as well as retinoic X receptor (RXR) in a ligand dependent manner. The amino terminal region of SRC-1 contains a PAS-A-basic helix-loop-helix homology domain which has previously been shown to be dimerization domains in other nuclear transcription factors including the aryl hydrocarbon (Ah) receptor and its heterodimerization partner, ARNT. The carboxy-terminal region of SRC-1 has been shown to possess histone acetyltransferase activity specific primarily for histones H3 and H4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: bHLHe74; Class E basic helix-loop-helix protein 74; Hin-2 protein; mNRC-1; NCoA-1; Nuclear receptor coactivator 1; Nuclear receptor coactivator protein 1; PAX3/NCOA1 fusion protein; Protein Hin-2; Renal carcinoma antigen NY-REN-52; RIP160; SRC-1; SRC-a/NCoA-1; Steroid receptor coactivator 1; Steroid Receptor Coactivator-1
Gene Aliases: bHLHe42; BHLHE74; F-SRC-1; KAT13A; NCOA1; RIP160; SRC-1; SRC1