Invitrogen

SRC1 Monoclonal Antibody (1135/H4)

Product Image Gallery

SRC1 Antibody — Published figure using SRC1 monoclonal antibody (Product # MA1-840)

SRC1 Antibody in Western Blot (WB) — Western blot analysis of SRC1 was performed by loading 25 µg of LNCaP (lane 1), PC-3 (lane 2) and NIH-3T3 (lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a SRC1 monoclonal antibody (Product # MA1-840) at a dilution of 1:2000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~152 and ~157 kDa.

Product Details

Tested Applications

Dilution

ChIP assay (ChIP)

5 µL / 10^6 cells

Flow Cytometry (Flow)

1 µg / 10^6 cells

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunoprecipitation (IP)

Assay dependent

Western Blot (WB)

1:500-1:5000

Published Applications

ChIP assay (ChIP)

See 2 publications below

Immunoprecipitation (IP)

See 2 publications below

Western Blot (WB)

See 8 publications below

Immunohistochemistry (IHC)

See 2 publications below

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Human, Mouse, Non-human primate, Rat

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1135/H4

Immunogen

Synthetic peptide corresponding to residues 477-947 of human SRC-1.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2150919

Product Specific Information

MA1-840 detects steroid receptor coactivator-1 (SRC-1) from mouse and primate tissues and cells as well as recombinant human SRC-1 protein.

MA1-840 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot MA1-840 detects an ~165 kDa protein representing SRC-1 in COS cell extract. Also by Western blot, MA1-840 detects an unidentified protein at ~30-40 kDa. This antibody does not react with NIH-3T3 cells in Western blot.

The MA1-840 immunogen is a synthetic peptide corresponding to residues 477-947 of human SRC-1. The epitope for MA1-840 has been mapped to the extreme C-terminal end of this fragment.

Target Information

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. One such coactivator is Steroid Receptor Coactivator-1 (SRC-1).

SRC-1 has been shown to interact with and stimulate the transcriptional activity of estrogen, progesterone, retinoic acid, thyroid and glucocorticoid receptors, as well as retinoic X receptor (RXR) in a ligand dependent manner. The amino terminal region of SRC-1 contains a PAS-A-basic helix-loop-helix homology domain which has previously been shown to be dimerization domains in other nuclear transcription factors including the aryl hydrocarbon (Ah) receptor and its heterodimerization partner, ARNT. The carboxy-terminal region of SRC-1 has been shown to possess histone acetyltransferase activity specific primarily for histones H3 and H4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: bHLHe74; Class E basic helix-loop-helix protein 74; Hin-2 protein; mNRC-1; NCoA-1; Nuclear receptor coactivator 1; Nuclear receptor coactivator protein 1; PAX3/NCOA1 fusion protein; Protein Hin-2; Renal carcinoma antigen NY-REN-52; RIP160; SRC-1; SRC-a/NCoA-1; Steroid receptor coactivator 1; Steroid Receptor Coactivator-1

Gene Aliases: bHLHe42; BHLHE74; F-SRC-1; KAT13A; NCOA1; RIP160; SRC-1; SRC1

UniProt ID: (Human) Q15788, (Mouse) P70365

Entrez Gene ID: (Human) 8648, (Mouse) 17977

Molecular Function: acetyltransferase transcription factor transferase