Invitrogen

SRC1 Monoclonal Antibody (1135/H4)

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SRC1 Antibody in Western Blot (WB) — Western blot analysis of SRC1 was performed by loading 25 µg of LNCaP (lane 1), PC-3 (lane 2) and NIH-3T3 (lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a SRC1 monoclonal antibody (Product # MA1-840) at a dilution of 1:2000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~152 and ~157 kDa.

SRC1 Antibody in Western Blot, Immunoprecipitation (WB, IP) — Figure 4. NHERF2 and SRC-1 form a complex to enhance ERalpha transactivation. ( A ) NHERF2 enhances the activity of the steroid receptor coactivator 1 (SRC-1). Control MCF7 cells and FLAG-NHERF2-MCF7 cells were transfected with pcDNA-SRC1 vector and ERalpha activity was determined by luciferase assay using the ERE-TK-Luc reporter. The cells were grown in the absence (white bars) or presence (gray bars) of E2 for 48 h. All results were normalized to luciferase activity observed in control MCF7 cells transfected with empty pcDNA vector ('Control'). Results were expressed as mean relative luciferase activity +- S.E. ( B ) NHERF2 knockdown impairs SRC1 coactivator activity on ERalpha transactivation. MCF7 cells were transfected with pcDNA-SRC1 in the presence of an NHERF2-specific siRNA (gray bar) or an unrelated control siRNA (white bars) as described in Materials and Methods. ERalpha activity was determined using the ERE-luciferase reporter in the presence of 100 nM E2. The results were normalized to luciferase activity of control MCF7 cells transfected with empty pcDNA vector and the unrelated control siRNA. ( C ) NHERF2 interacts with ERalpha and SRC-1 in ZR-75-1 breast cancer cells. Whole cell protein extracts were subjected to IP using anti-ERalpha (upper panel), anti-SRC-1 (lower panel) followed by immunoblot using anti-NHERF2, anti-ER or anti-SRC-1 antibodies. As a negative control immunoprecipitation was performed using anti-IgG (IgG, upper and lower panels). (

SRC1 Antibody in ChIP assay (ChIP) — Published figure using SRC1 monoclonal antibody (Product # MA1-840) in ChIP assay

Product Details

MA1-840

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:5,000	View 8 publications 8 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:200	-
Flow Cytometry (Flow)	1 µg / 10^6 cells	-
Immunoprecipitation (IP)	Assay-dependent	View 3 publications 3 publications
ChIP assay (ChIP)	5 µL / 10^6 cells	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse, Non-human primate
Published species	Human, Mouse, Non-human primate, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	1135/H4
Immunogen	Synthetic peptide corresponding to residues 477-947 of human SRC-1. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	purified
Storage buffer	PBS with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2150919

Product Specific Information

MA1-840 detects steroid receptor coactivator-1 (SRC-1) from mouse and primate tissues and cells as well as recombinant human SRC-1 protein.

MA1-840 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot MA1-840 detects an ~165 kDa protein representing SRC-1 in COS cell extract. Also by Western blot, MA1-840 detects an unidentified protein at ~30-40 kDa. This antibody does not react with NIH-3T3 cells in Western blot.

The MA1-840 immunogen is a synthetic peptide corresponding to residues 477-947 of human SRC-1. The epitope for MA1-840 has been mapped to the extreme C-terminal end of this fragment.

Target Information

Clone 1135 recognizes SRC-1, steroid-receptor coactivator-1. SRC-1 is a member of a gene family that functions in the full transcriptional activation of the steroid hormone receptor superfamily and contains intrinsic histone acetyltransferase activity. SRC-1 has a molecular weight of 160 kDa and has a basic helix-loop-helix (bHLH)-PAS domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nuclear receptor corepressors Ncor1 and Ncor2 (Smrt) are required for retinoic acid-dependent repression of Fgf8 during somitogenesis.

Developmental biology

Kumar S,Cunningham TJ,Duester G

MA1840 was used in ChIP assay to propose a model in which NCOR1/2 mediates retinoic acid-dependent repression of Fgf8 in caudal progenitors

Sat Oct 01 00:00:00 EDT 2016

SIP1/NHERF2 enhances estrogen receptor alpha transactivation in breast cancer cells.

Nucleic acids research

Meneses-Morales I,Tecalco-Cruz AC,Barrios-García T,Gómez-Romero V,Trujillo-González I,Reyes-Carmona S,García-Zepeda E,Méndez-Enríquez E,Cervantes-Roldán R,Pérez-Sánchez V,Recillas-Targa F,Mohar-Betancourt A,León-Del-Río A

MA1-840 was used in immunoprecipitation and western blot to study the role of NHERF2 as an ER-alpha co-activator in breast cancer cells

Sun Jun 01 00:00:00 EDT 2014

SIP1/NHERF2 enhances estrogen receptor alpha transactivation in breast cancer cells.

Nucleic acids research

MA1-840 was used in immunoprecipitation and western blot to study the role of NHERF2 as an ER-alpha co-activator in breast cancer cells

Sun Jun 01 00:00:00 EDT 2014

D538G mutation in estrogen receptor-α: A novel mechanism for acquired endocrine resistance in breast cancer.

Cancer research

Merenbakh-Lamin K,Ben-Baruch N,Yeheskel A,Dvir A,Soussan-Gutman L,Jeselsohn R,Yelensky R,Brown M,Miller VA,Sarid D,Rizel S,Klein B,Rubinek T,Wolf I

MA1-840 was used in immunoprecipitation to study the role of ER-alpha D538G mutation in mediating aquired resistance to hormone therapy in breast cancer patients

Sun Dec 01 00:00:00 EST 2013

Expression patterns of the steroid receptor coactivator family in human ovarian endometriosis.

The journal of obstetrics and gynaecology research

Kumagami A,Ito A,Yoshida-Komiya H,Fujimori K,Sato A

MA1-840 was used in immunohistochemistry to characterize the expression of estrogen and steroid receptor cofactors in human endometriosis

Sat Oct 01 00:00:00 EDT 2011

Constitutive activation of the mitogen-activated protein kinase pathway impairs vitamin D signaling in human prostate epithelial cells.

Journal of cellular physiology

Zhang Z,Kovalenko P,Cui M,Desmet M,Clinton SK,Fleet JC

MA1-840 was used in western blot to investigate the effect of constitutive activation of MAPK signaling on vitamin D signaling in immortalized human prostate epithelial cells

Sun Aug 01 00:00:00 EDT 2010

Role of steroid receptor coactivators in glucocorticoid and transforming growth factor beta regulation of plasminogen activator inhibitor gene expression.

Molecular endocrinology (Baltimore, Md.)

Li G,Heaton JH,Gelehrter TD

MA1-840 was used in western blot to study the regulation of plasminogen activator inhibitor gene expression through steroid receptor coactivators.

Mon May 01 00:00:00 EDT 2006

Role of SRC-1 in the promotion of prostate cancer cell growth and tumor progression.

Cancer research

Agoulnik IU,Vaid A,Bingman WE,Erdeme H,Frolov A,Smith CL,Ayala G,Ittmann MM,Weigel NL

MA1-840 was used in immunohistochemistry to study the role of SRC-1 in prostate cancer cell growth and tumor progression.

Thu Sep 01 00:00:00 EDT 2005

Nuclear coactivator-62 kDa/Ski-interacting protein is a nuclear matrix-associated coactivator that may couple vitamin D receptor-mediated transcription and RNA splicing.

The Journal of biological chemistry

Zhang C,Dowd DR,Staal A,Gu C,Lian JB,van Wijnen AJ,Stein GS,MacDonald PN

MA1-840 was used in chromatin immunoprecipitation to investigate the role of nuclear coactivator-62 kDa/Ski-interacting protein (NCoA62/SKIP) during the vitamin D receptor (VDR)-mediated transcription and RNA splicing.

Fri Sep 12 00:00:00 EDT 2003

Ordered recruitment of histone acetyltransferases and the TRAP/Mediator complex to thyroid hormone-responsive promoters in vivo.

Proceedings of the National Academy of Sciences of the United States of America

Sharma D,Fondell JD

MA1-840 was used in western blot to study the role of histone acetylation in TRAP/Mediator complex recruitment and function.

Tue Jun 11 00:00:00 EDT 2002

Endogenously expressed estrogen receptor and coactivator AIB1 interact in MCF-7 human breast cancer cells.

Proceedings of the National Academy of Sciences of the United States of America

Tikkanen MK,Carter DJ,Harris AM,Le HM,Azorsa DO,Meltzer PS,Murdoch FE

MA1-840 was used in western blot to demonstrate the role of AIB1 in human estrogen receptor signal pathway in MCF-7 human breast cancer cells.

Tue Nov 07 00:00:00 EST 2000

Endogenously expressed estrogen receptor and coactivator AIB1 interact in MCF-7 human breast cancer cells.

Proceedings of the National Academy of Sciences of the United States of America

Tikkanen MK,Carter DJ,Harris AM,Le HM,Azorsa DO,Meltzer PS,Murdoch FE

MA1-840 was used in western blot to demonstrate the role of AIB1 in human estrogen receptor signal pathway in MCF-7 human breast cancer cells.

Tue Nov 07 00:00:00 EST 2000

Recruitment of nuclear receptor corepressor and coactivator to the retinoic acid receptor by retinoid ligands. Influence of DNA-heterodimer interactions.

The Journal of biological chemistry

Klein ES,Wang JW,Khalifa B,Gavigan SA,Chandraratna RA

MA1-840 was used in western blot to study the activation mechanism for retinoic acid receptors by their ligands.

Fri Jun 23 00:00:00 EDT 2000

Recruitment of nuclear receptor corepressor and coactivator to the retinoic acid receptor by retinoid ligands. Influence of DNA-heterodimer interactions.

The Journal of biological chemistry

Klein ES,Wang JW,Khalifa B,Gavigan SA,Chandraratna RA

MA1-840 was used in western blot to study the activation mechanism for retinoic acid receptors by their ligands.

Fri Jun 23 00:00:00 EDT 2000

Partial hormone resistance in mice with disruption of the steroid receptor coactivator-1 (SRC-1) gene.

Science (New York, N.Y.)

Xu J,Qiu Y,DeMayo FJ,Tsai SY,Tsai MJ,O'Malley BW

MA1-840 was used in western blot to investigate the functional properties of SRC-1 in mice

Fri Mar 20 00:00:00 EST 1998

Steroid receptor coactivator-1 is a histone acetyltransferase.

Nature

Spencer TE,Jenster G,Burcin MM,Allis CD,Zhou J,Mizzen CA,McKenna NJ,Onate SA,Tsai SY,Tsai MJ,O'Malley BW

MA1-840 was used in western blot to characterize the steroid receptor coactivator 1

Thu Sep 11 00:00:00 EDT 1997

Bioinformatics

Protein Aliases: ASV; bHLHe74; c-SRC; Class E basic helix-loop-helix protein 74; Hin-2 protein; mNRC-1; NCoA-1; Nuclear receptor coactivator 1; Nuclear receptor coactivator protein 1; PAX3/NCOA1 fusion protein; Protein Hin-2; Renal carcinoma antigen NY-REN-52; RIP160; SRC-1; SRC-1 (-Q); SRC-a/NCoA-1; Steroid receptor coactivator 1; steroid receptor coactivator-1

Gene Aliases: bHLHe42; BHLHE74; F-SRC-1; KAT13A; NCOA1; RIP160; SRC-1; SRC1

UniProt ID: (Human) Q15788, (Mouse) P70365

Entrez Gene ID: (Human) 8648, (Mouse) 17977

Molecular Function: histone modifying enzyme