Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:5,000 | View 8 publications 8 publications |
Immunohistochemistry (IHC) |
- | View 2 publications 2 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:200 | - |
Flow Cytometry (Flow) |
1 µg / 10^6 cells | - |
Immunoprecipitation (IP) |
Assay-dependent | View 3 publications 3 publications |
ChIP assay (ChIP) |
5 µL / 10^6 cells | View 2 publications 2 publications |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Non-human primate |
Published species |
Human, Mouse, Non-human primate, Rat |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
1135/H4 |
Immunogen |
Synthetic peptide corresponding to residues 477-947 of human SRC-1. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
purified |
Storage buffer |
PBS with 1mg/mL BSA |
Contains |
0.05% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2150919 |
MA1-840 detects steroid receptor coactivator-1 (SRC-1) from mouse and primate tissues and cells as well as recombinant human SRC-1 protein.
MA1-840 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot MA1-840 detects an ~165 kDa protein representing SRC-1 in COS cell extract. Also by Western blot, MA1-840 detects an unidentified protein at ~30-40 kDa. This antibody does not react with NIH-3T3 cells in Western blot.
The MA1-840 immunogen is a synthetic peptide corresponding to residues 477-947 of human SRC-1. The epitope for MA1-840 has been mapped to the extreme C-terminal end of this fragment.
Clone 1135 recognizes SRC-1, steroid-receptor coactivator-1. SRC-1 is a member of a gene family that functions in the full transcriptional activation of the steroid hormone receptor superfamily and contains intrinsic histone acetyltransferase activity. SRC-1 has a molecular weight of 160 kDa and has a basic helix-loop-helix (bHLH)-PAS domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ASV; bHLHe74; c-SRC; Class E basic helix-loop-helix protein 74; Hin-2 protein; mNRC-1; NCoA-1; Nuclear receptor coactivator 1; Nuclear receptor coactivator protein 1; PAX3/NCOA1 fusion protein; Protein Hin-2; Renal carcinoma antigen NY-REN-52; RIP160; SRC-1; SRC-1 (-Q); SRC-a/NCoA-1; Steroid receptor coactivator 1; steroid receptor coactivator-1
Gene Aliases: bHLHe42; BHLHE74; F-SRC-1; KAT13A; NCOA1; RIP160; SRC-1; SRC1
UniProt ID: (Human) Q15788, (Mouse) P70365
Entrez Gene ID: (Human) 8648, (Mouse) 17977
Molecular Function:
histone modifying enzyme
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