Immunohistochemistry was performed on normal biopsies of deparaffinized Human pancreas tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a SRC3 monoclonal antibody (Product # MA1-845) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||GST fusion protein encoding amino acid residues 605-1294 of human AIB1.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||2µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-845 detects amplified in breast cancer-1 (AIB1) in human cells.
MA1-845 has been successfully used in Western blot and immunoprecipitation, ChIP, and immunohistochemistry procedures. By Western blot, this antibody detects an ~155 kDa protein representing AIB1 from MCF-7 cell extract.
The MA1-845 immunizing peptide corresponds to amino acid residues 605-1294 of human AIB1.
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Amplified in breast cancer-1 (AIB-1), is a member of a growing family of transcriptional coactivator proteins which includes steroid receptor coactivator-1 (SRC-1) and glucocorticoid receptor interacting protein-1 (GRIP1. AIB1/RAC3 has been shown to interact and stimulate transcriptional activity of the retinoic acid, retinoid X, vitamin D, mineralocorticoid, glucocorticoid, thyroid hormone, androgen, progesterone, and peroxisome proliferator activated receptors. AIB1/RAC3 also interacts with the transcriptional integrators CREB binding protein (CBP) and p300.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Notch-1 activates estrogen receptor-alpha-dependent transcription via IKKalpha in breast cancer cells.
MA1-845 was used in ChIP assay to study the role of IKKalpha in the activation of estrogen receptor-alpha driven transcription by Notch-1
|Hao L,Rizzo P,Osipo C,Pannuti A,Wyatt D,Cheung LW,Sonenshein G,Osborne BA,Miele L||Oncogene (29:201)||2010|
Progressive loss of estrogen receptor alpha cofactor recruitment in endocrine resistance.
MA1-845 was used in western blot to study the mechanism for endocrine resistance in breast cancer
|Naughton C,MacLeod K,Kuske B,Clarke R,Cameron DA,Langdon SP||Molecular endocrinology (Baltimore, Md.) (21:2615)||2007|
Two distinct coactivators, DRIP/mediator and SRC/p160, are differentially involved in vitamin D receptor transactivation during keratinocyte differentiation.
MA1-845 was used in western blot to study the roles of DRIP/mediator and SRC/p160 during the early stages of differentiation.
|Oda Y,Sihlbom C,Chalkley RJ,Huang L,Rachez C,Chang CP,Burlingame AL,Freedman LP,Bikle DD||Molecular endocrinology (Baltimore, Md.) (17:2329)||2003|