|Tested species reactivity||Human, Mouse, Rat, Dog, Chicken, Hamster|
|Published species reactivity||Avian, Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||6 His-tag fusion protein of SREBP1 corresponding to amino acids 301-407|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western blot, a band can be seen at 125 kDa (precursor) and additional bands may be seen at 60-70 kDa (cleaved).
Suggested positive control: IMR 5 and HeLa cells..
The importance of lipids for survival is underscored when starvation occurs – cellular responses occur in major organ pathways to protect against detrimental loss of lipids. For example, lipid generation and stabilization occurs in the brain even when serious deficiencies of dietary lipid exist. Embryonic and fetal tissues also are spared the effects of lacking lipids, even at the expense of maternal health. In all of these examples, the transcription factor Sterol-Regulatory Element-Binding Protein 1 (SREBP1) is a critical contributor to effective cholesterol and fatty acid synthesis, through the PPAR-gamma and AMPK signaling pathways. During fasting and starvation, more than 40% of transcription factors in an individual are dedicated to adipogenesis. However, in certain conditions that lead to obesity, the SREBP1 transcription activity is overstimulated by elevated cofactor expression (as in the case of resistin) and the result is erroneous de novo lipogenesis in hepatocytes and plasma. Excessive lipogenesis in these tissues is then associated with dyslipidemia, atherosclerosis and abnormal HDL/LDL/VLDL ratios and commonly attributed obesity-related disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The epigenetic drug 5-azacytidine interferes with cholesterol and lipid metabolism.
MA5-16124 was used in western blot to study the DNA methylation-independent mechanism by which the epigenetic drug 5-azacytidine inhibits the expression of genes involved in cholesterol and lipid metabolism
|Poirier S,Samami S,Mamarbachi M,Demers A,Chang TY,Vance DE,Hatch GM,Mayer G||The Journal of biological chemistry (289:18736)||2014|
PPAR¿ activation attenuates hepatic steatosis in Ldlr-/- mice by enhanced fat oxidation, reduced lipogenesis, and improved insulin sensitivity.
MA5-16124 was used in western blot to study the roles of decreased lipogenesis, increased beta-oxidation and enhanced insulin-sensitivity in the mechanism by which PPAR-delta protects against hepatic steatosis in LDLR-deficient mice
|Bojic LA,Telford DE,Fullerton MD,Ford RJ,Sutherland BG,Edwards JY,Sawyez CG,Gros R,Kemp BE,Steinberg GR,Huff MW||Journal of lipid research (55:1254)||2014|
|Not Applicable||Not Cited||
Lack of liver X receptors leads to cell proliferation in a model of mouse dorsal prostate epithelial cell.
MA5-16124 was used in western blot to study altered signaling pathways in prostate cells isolated from Lxralphabeta knockout mice
|Dufour J,Pommier A,Alves G,De Boussac H,Lours-Calet C,Volle DH,Lobaccaro JM,Baron S||PloS one (8:null)||2013|
Effect of cholesterol on lipogenesis and VLDL-TG assembly and secretion in goose primary hepatocytes.
MA5-16124 was used in western blot to study lipogenesis and the assembly and secretion of VLDL-triglyceride in goose primary hepatocytes and the effects of different concentrations of cholesterol
|Han CC,Wang JW,Pan ZX,Tang H,Xiang SX,Wang J,Li L,Xu F,Wei SH||Molecular and cellular biochemistry (374:163)||2013|
Inhibition of intestinal bile acid transporter Slc10a2 improves triglyceride metabolism and normalizes elevated plasma glucose levels in mice.
MA5-16124 was used in western blot to study the beneficial changes in triglyceride metabolism and plasma glucose concentrations of ob/ob obese mice following inhibition of intestinal Slc10a2
|Lundåsen T,Andersson EM,Snaith M,Lindmark H,Lundberg J,Östlund-Lindqvist AM,Angelin B,Rudling M||PloS one (7:null)||2012|
The lipogenic transcription factor ChREBP dissociates hepatic steatosis from insulin resistance in mice and humans.
MA5-16124 was used in western blot to study the role of the lipogenic transcription factor ChREBP in hepatic steatosis and insulin resistance in humans and mice
|Benhamed F,Denechaud PD,Lemoine M,Robichon C,Moldes M,Bertrand-Michel J,Ratziu V,Serfaty L,Housset C,Capeau J,Girard J,Guillou H,Postic C||The Journal of clinical investigation (122:2176)||2012|
Glucose 6-phosphate, rather than xylulose 5-phosphate, is required for the activation of ChREBP in response to glucose in the liver.
MA5-16124 was used in western blot to study the role of glucose-6-phosphate as the glucose-signaling metabolite that activates ChREBP in response to hepatic glucose
|Dentin R,Tomas-Cobos L,Foufelle F,Leopold J,Girard J,Postic C,Ferré P||Journal of hepatology (56:199)||2012|
Distinct regulation of adiponutrin/PNPLA3 gene expression by the transcription factors ChREBP and SREBP1c in mouse and human hepatocytes.
MA5-16124 was used in western blot to study the differential roles of ChREBP and SREBP1c in regulating adiponutrin expression in mouse and human hepatocytes
|Dubuquoy C,Robichon C,Lasnier F,Langlois C,Dugail I,Foufelle F,Girard J,Burnol AF,Postic C,Moldes M||Journal of hepatology (55:145)||2011|
The TRC8 ubiquitin ligase is sterol regulated and interacts with lipid and protein biosynthetic pathways.
MA5-16124 was used in western blot to study the role of sterol-regulated TRC8 ubiquitin ligase in regulation of lipid and protein biosynthetic pathways
|Lee JP,Brauweiler A,Rudolph M,Hooper JE,Drabkin HA,Gemmill RM||Molecular cancer research : MCR (8:93)||2010|
Regulation of energy substrate utilization and hepatic insulin sensitivity by phosphatidylcholine transfer protein/StarD2.
MA5-16124 was used in western blot to examine the role of phosphatidylcholine transfer protein/StarD2 in hepatic insulin sensitivity
|Scapa EF,Pocai A,Wu MK,Gutierrez-Juarez R,Glenz L,Kanno K,Li H,Biddinger S,Jelicks LA,Rossetti L,Cohen DE||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (22:2579)||2008|
Liver-specific inhibition of ChREBP improves hepatic steatosis and insulin resistance in ob/ob mice.
MA5-16124 was used in western blot to study the beneficial effects of shRNA-mediated knockdown of ChREBP on hepatic steatosis and insulin sensitivity in ob/ob leptin-deficient mice
|Dentin R,Benhamed F,Hainault I,Fauveau V,Foufelle F,Dyck JR,Girard J,Postic C||Diabetes (55:2159)||2006|
Insulin regulation of glucokinase gene expression: evidence against a role for sterol regulatory element binding protein 1 in primary hepatocytes.
MA5-16124 was used in western blot to study the lack of SREBP-1 involvement in the regulation of glucokinase gene expression in primary hepatocytes
|Gregori C,Guillet-Deniau I,Girard J,Decaux JF,Pichard AL||FEBS letters (580:410)||2006|
Polyunsaturated fatty acids suppress glycolytic and lipogenic genes through the inhibition of ChREBP nuclear protein translocation.
MA5-16124 was used in western blot to study the mechanism by which polyunsaturated fatty acids suppress the expresson of genes involved in glycolysis and lipogenesis
|Dentin R,Benhamed F,Pégorier JP,Foufelle F,Viollet B,Vaulont S,Girard J,Postic C||The Journal of clinical investigation (115:2843)||2005|
Hepatic farnesyl diphosphate synthase expression is suppressed by polyunsaturated fatty acids.
MA5-16124 was used in western blot to study the mechanism by which polyunsaturated fatty acids suppress liver farnesyl diphosphate synthase expression
|Le Jossic-Corcos C,Gonthier C,Zaghini I,Logette E,Shechter I,Bournot P||The Biochemical journal (385:787)||2005|
Hepatic glucokinase is required for the synergistic action of ChREBP and SREBP-1c on glycolytic and lipogenic gene expression.
MA5-16124 was used in western blot to study the role of hepatic glucokinase in the synergistic action of ChREBP and SREBP-1c on glycolytic and lipogenic gene expression
|Dentin R,Pégorier JP,Benhamed F,Foufelle F,Ferré P,Fauveau V,Magnuson MA,Girard J,Postic C||The Journal of biological chemistry (279:20314)||2004|
Evaluation of Fbxw7 expression and its correlation with expression of SREBP-1 in a mouse model of NAFLD.
MA5-16124 was used in immunohistochemistry to study the relationship between Fbxw7 and SREBP-1 in a murine model of non-alcoholic fatty liver disease
|Tu K,Zheng X,Yin G,Zan X,Yao Y,Liu Q||Molecular medicine reports (6:525)||2012|