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Western Blot staining of HEK293 lysate overexpressing Human TRIM21 (c-term Myc tagged and loaded in Lane A&C) stained with PA5-18147 at a concentration of 1 ug/ml (lane A). Lane C was stained with anti-MYC Tag at a dilution of (1/1000). Lane B was loaded with mock-transfected HEK293 cells and stained using PA5-18147 at a concentration of 1 mg/ml. Primary antibody incubation was performed for 1 hour and detection was performed by chemiluminescence.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide sequence (CPLNIGSQGSTDY) corresponding to the C-terminus amino acids of TRIM21|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.2 - 1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes a member of the tripartite motif family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
52 kDa ribonucleoprotein autoantigen Ro/SS-A; 52 kDa Ro protein; 52kD Ro/SSA autoantigen; 52kDa ribonucleoprotein autoantigen SS-A/Ro; RING finger protein 81; RNF81, RO52, SSA, SSA1; ro(SS-A); Sicca syndrome antigen A; sjoegren syndrome type A antigen; Sjogren syndrome antigen A1 (52kD, ribonucleoprotein autoantigen SS-A/Ro); Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro); SS-A; TRIM21; tripartite motif protein 21; tripartite motif protein TRIM21; tripartite motif-containing 21; tripartite motif-containing protein 21
RNF81; Ro/SSA; RO52; SSA; SSA1; TRIM21