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Immunofluorescent analysis of SSEA-1 (green) showing membrane staining of F9 cells (right panel) compared to negative undifferentiated NCCIT control cells (left panel). The cells were fixed with formalin for 15 minutes, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 488-conjugated SSEA-1 monoclonal antibody (Product # MA1-022-D488) in 3% BSA-PBS at a dilution of 1:20 and incubated for 1 hour at 37C in the dark. Nuclei (both panels, blue) were stained with DAPI. Images were taken at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgM|
|Immunogen||BALB/c mouse immunized with F9 teratocarcinoma stem cells (X-irradiated).|
|Purification||Affinity chromatography - MBP|
|Storage buffer||PBS with proprietary stabilizer|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-022-D488 has been successfully used in ICC/IF and flow cytometry applications on mouse and human samples.
SSEA-1 (stage-specific embryonic antigen-1) is a cell surface antigen, expressed in murine embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.