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Flow cytometry analysis of SSEA-1 in F9 (red histogram) and NCCIT cells (orange histogram) compared to unstained cells (blue histogram). Positive staining is observed on F9 cells when compared to no antibody control (middle panel) and to SSEA-1-negative undifferentiated NCCIT cells (right panel). Cells were fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with 2% BSA-PBS for 30 minutes at room temperature and incubated with a DyLight 550-conjugated SSEA-1 monoclonal antibody (Product # MA1-022-D550) in 2% BSA-PBS at a dilution of 1:100 for 60 minutes at room temperature. Cells were washed and re-suspended in PBS for FACS analysis.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgM|
|Immunogen||BALB/c mouse immunized with F9 teratocarcinoma stem cells (X-irradiated).|
|Purification||Affinity chromatography - MBP|
|Storage buffer||PBS with proprietary stabilizer|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-022-D550 has been successfully used in flow cytometry on mouse and human samples.
SSEA-1 (stage-specific embryonic antigen-1) is a cell surface antigen, expressed in murine embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.