Immunofluorescent analysis of SSEA4 using anti-SSEA4 monoclonal antibody (Product# MA1-021) shows expression in human teratocarcinoma NCCIT cells (shown in green) but not in negative control HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product #37525) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing SSEA4 (Product# MA1-021), at a dilution of 1:50 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody (Product# 35503) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG3|
|Immunogen||human embryonal carcinoma cell line 2102Ep|
|Storage buffer||potassium phosphate with 0.15M NaCl, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-021X has been successfully used in immunofluorescence and flow cytometry applications on human samples.
Stage-specific embryonic antigen 4 (SSEA-4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Amnion Epithelial Cells of Buffalo (Bubalus Bubalis) Term Placenta Expressed Embryonic Stem Cells Markers and Differentiated into Cells of Neurogenic Lineage In Vitro.
MA1-021X was used in immunocytochemistry to isolate and characterize buffalo amniotic membrane-derived epithelial cells
|Ghosh K,Selokar NL,Gahlawat SK,Kumar D,Kumar P,Yadav PS||Animal biotechnology (27:38)||2015|
|Not Applicable||Not Cited||
Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells.
MA1-021X was used in immunocytochemistry to use MC631 and MC813-70 to study glycolipids in human teratocarcinoma
|Kannagi R,Cochran NA,Ishigami F,Hakomori S,Andrews PW,Knowles BB,Solter D||The EMBO journal (2:2355)||1984|