Immunofluorescent analysis of SSEA-5 in NCCIT (3 left bars) and negative control HeLa cells (3 right bars) in a 96 well plate assay. Formalin-fixed cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a DyLight 755-conjugated SSEA-5 monoclonal antibody (Product # MA1-144-D755) in 1% BSA-PBS at a dilution of 1:100 for 1 hour at room temperature, washed with PBS, and fluorescence intensity was measured at 800nm using a near-IR imaging system. Fluorescence intensities from 3 independent wells for each cell line grown to approximately 80% confluence are shown.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Undifferentiated H9 human embryonic stem cells|
|Storage buffer||PBS with proprietary stabilizer|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Immunofluorescent analysis of MA1-144-D755 shows positive staining of SSEA-5 in human embryonal carcinoma NCCIT cells.
Stage-Specific Embryonic Antigen-5 (SSEA-5) is an oligosaccharide that can be part of glycosphingolipids and glycoproteins. It contains the motif Fuc1-2Gal-beta1-3GlcNAc-beta, which is characteristic of H-1 antigens. SSEA-5 is found in the inner cell mass of the human blastocyst during embryogenesis, on undifferentiated human embryonic stem cells and on the surface of induced pluripotent cells. SSEA-5 was found to proceed the loss of other known stem cell markers and the combination of SSEA-5 with either CD9 and CD90 or CD50 and CD200 was superior in identifying and eliminating rare undifferentiated human ES cells from differentiated cell populations than the use of other established stem cell markers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.