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Immunofluorescent analysis of SSEA-1 using anti-SSEA-1 monoclonal antibody (Product# MA1-022) shows specific expression in mouse testicular teratoma F9 cells (shown in green) but not in negative control human NTERA-2 teratocarcinoma cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product #37525) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing SSEA-1 (Product# MA1-022), at a dilution of 1:50 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with a fluorescent dye labeled goat-anti-mouse IgM secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgM|
|Immunogen||BALB/c mouse immunized with F9 teratocarcinoma stem cells (X-irradiated).|
|Purification||Affinity chromatography - MBP|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100|
|Immunohistochemistry (Frozen) (IHC (F))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
SSEA-1 (stage-specific embryonic antigen-1) is a cell surface antigen, expressed in murine embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CD15; MC-480; SSEA-1; stage-specific embryonic antigen 1