Invitrogen

SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), Biotin, eBioscience™

Product Image Gallery

SSEA1 Antibody in Flow Cytometry (Flow) — Staining of the F9 cell line with staining buffer (autofluorescence) (open histogram) or 0.25 µg of Anti-Human/Mouse SSEA-1 Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

SSEA1 Antibody in Immunocytochemistry (ICC/IF) — Published figure using SSEA1 monoclonal antibody (Product # 13-8813-82) in Immunocytochemistry

SSEA1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Published figure using SSEA1 monoclonal antibody (Product # 13-8813-82) in Immunocytochemistry and Immunohistochemistry

SSEA1 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow) — Extended Data Figure 10 Disruption ofDisruption of Jag2 in basal stem/progenitor cells causes the differentiation of secretory progenitor cells into ciliated cells without affecting proliferation or apoptosis a, Schematic representation of Jag2 inhibition using lentiviruses (LV) carrying shRNAs. Infected GFP + cells were cultured in an air-liquid interface (ALI) culture system for 23d, when they were harvested, sorted and analyzed. b, Relative mRNA expression of Jag2 in tracheal epithelial cells infected with mock vector (control) or with vectors carrying 4 different shRNAs targeting Jag2 72h after infection. c, Relative mRNA expression of Jag2 in tracheal epithelial basal cells infected with mock vector (control) or with lentivirus targeting Jag2 (shJag2 877) after 23d in ALI. d, Relative mRNA expression of the secretory genes ( Scgb1a1 and Scgb3a2 ) and the ciliated cell genes ( FoxJ1 and c-Myb ) in mock (black bars) and shJag2 877 (grey bars) infected cells 23d after ALI initiation. Relative expression is normalized to baseline transcript levels in mock infected cells. e, Relative mRNA expression of Jag2 on sorted recombined (YFP + ) basal cells and unrecombined YFP - basal cells from Tam-treated CK5-Jag2 fl/fl mice (n=3 mice). Relative expression is normalized to baseline transcript levels in YFP - cells. f, Percentage of YFP + cells per total DAPI + cells (efficiency of recombination) on either Tam-treated CK5-Jag2 +/+ control (black bars) or Tam-treated CK5-Jag2 fl/fl (

SSEA1 Antibody in Immunocytochemistry, Immunohistochemistry, Flow Cytometry (ICC/IF, IHC, Flow) — Figure 2 Characterization of pluripotency in iPSC clones reprogrammed upon Ezh2 inactivation. A. Images of phase contrast (left), alkaline phosphatase staining (middle) and Oct4 -driven GFP fluorescence (right) of representative control ( Ezh2 +/DeltaSET , upper row) and mutant ( Ezh2 DeltaSET/DeltaSET , lower row) iPSC colonies. B. Flow cytometric analysis of SSEA-1 and endogenous OCT4 (OCT4-GFP) levels in representative control ( Ezh2 +/DeltaSET , upper row) and mutant ( Ezh2 DeltaSET/DeltaSET , lower row) iPSC clones (cl.1 and cl.2). Embryonic stem cells (ESC) were used as positive control for SSEA1 expression (upper left). C. Growth curve of representative control ( Ezh +/+ , grey) and mutant ( Ezh2 DeltaSET/DeltaSET , purple) iPSC clones cultured in 2i/LIF medium for the indicated hours. Column height represents cell number. D. Hematoxylin & eosin staining and immunohistochemical analysis of representative sections of teratomas generated from either Ezh2 control ( Ezh2 +/+ ) or mutant ( Ezh2 DeltaSET/DeltaSET ) iPSC cells of two representative clones. Stainings for mesoderm (upper row), endoderm (middle row) and ectoderm (lower row) markers are displayed. Data displayed in A, B, C and D are representative of at least four independent experiments, using two iPSC clones per genotype. E. Scatter plots showing global gene expression correlation analyses between Ezh2 +/+ and Ezh2 DeltaSET/DeltaSET iPSC (left panel), Ezh2 +/+ iPSCs and MEF (central panel) and Ezh2 DeltaSET/Del

SSEA1 Antibody in Immunohistochemistry (IHC) — Published figure using SSEA1 monoclonal antibody (Product # 13-8813-82) in Immunohistochemistry

Product Details

13-8813-82

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 4 publications 4 publications
Immunocytochemistry (ICC/IF)	-	View 8 publications 8 publications
Flow Cytometry (Flow)	0.5 µg/test	View 12 publications 12 publications
Functional Assay (FN)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse
Host/Isotype	Mouse / IgM
Class	Monoclonal
Type	Antibody
Clone	eBioMC-480 (MC-480)
Conjugate	Biotin Alexa Fluor 488 eFluor 660 PE PerCP-eFluor 710 Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, do not freeze
RRID	AB_657625

Product Specific Information

Description: The eBioMC-480 (MC-480) antibody reacts with the stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine and human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. Expression of the carbohydrate moiety is also found on mature human granulocytes (on CD15) and some monocytes. SSEA-1 is associated with cell adhesion, migration and differentiation.

Applications Reported: This eBioMC-480 (MC-480) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioMC-480 (MC-480) antibody has been tested by flow cytometric analysis of the F9 cell line. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

BAF complex-mediated chromatin relaxation is required for establishment of X chromosome inactivation.

Nature communications

Keniry A,Jansz N,Gearing LJ,Wanigasuriya I,Chen J,Nefzger CM,Hickey PF,Gouil Q,Liu J,Breslin KA,Iminitoff M,Beck T,Tapia Del Fierro A,Whitehead L,Jarratt A,Kinkel SA,Taberlay PC,Willson T,Pakusch M,Ritchie ME,Hilton DJ,Polo JM,Blewitt ME

13-8813-82 was used in Functional assay to report a replenishable female mouse embryonic stem cell (mESC) system, Xmas, that allows rapid assessment of X chromosome inactivation (XCI), the epigenetic silencing mechanism of one of the two X chromosomes that enables dosage compensation in female mammals.

Tue Mar 29 00:00:00 EDT 2022

High-throughput screening in postimplantation haploid epiblast stem cells reveals Hs3st3b1 as a modulator for reprogramming.

Stem cells translational medicine

Gao Q,Zhang W,Zhao Y,Tian Y,Wang Y,Zhang J,Geng M,Xu M,Yao C,Wang H,Li L,Liu Y,Shuai L

Published figure using SSEA1 monoclonal antibody (Product # 13-8813-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

ECE2 regulates neurogenesis and neuronal migration during human cortical development.

EMBO reports

Buchsbaum IY,Kielkowski P,Giorgio G,O'Neill AC,Di Giaimo R,Kyrousi C,Khattak S,Sieber SA,Robertson SP,Cappello S

Published figure using SSEA1 monoclonal antibody (Product # 13-8813-82) in Flow Cytometry

Wed May 06 00:00:00 EDT 2020

A vital sugar code for ricin toxicity.

Cell research

Taubenschmid J,Stadlmann J,Jost M,Klokk TI,Rillahan CD,Leibbrandt A,Mechtler K,Paulson JC,Jude J,Zuber J,Sandvig K,Elling U,Marquardt T,Thiel C,Koerner C,Penninger JM

Published figure using SSEA1 monoclonal antibody (Product # 13-8813-82) in Flow Cytometry

Wed Nov 01 00:00:00 EDT 2017

A vital sugar code for ricin toxicity.

Cell research

Taubenschmid J,Stadlmann J,Jost M,Klokk TI,Rillahan CD,Leibbrandt A,Mechtler K,Paulson JC,Jude J,Zuber J,Sandvig K,Elling U,Marquardt T,Thiel C,Koerner C,Penninger JM

Published figure using SSEA1 monoclonal antibody (Product # 13-8813-82) in Flow Cytometry

Wed Nov 01 00:00:00 EDT 2017

Comparative glycoproteomics of stem cells identifies new players in ricin toxicity.

Nature

Stadlmann J,Taubenschmid J,Wenzel D,Gattinger A,Dürnberger G,Dusberger F,Elling U,Mach L,Mechtler K,Penninger JM

Published figure using SSEA1 monoclonal antibody (Product # 13-8813-82) in Immunocytochemistry

Thu Sep 28 00:00:00 EDT 2017

Noncanonical function of DGCR8 controls mESC exit from pluripotency.

The Journal of cell biology

Cirera-Salinas D,Yu J,Bodak M,Ngondo RP,Herbert KM,Ciaudo C

Published figure using SSEA1 monoclonal antibody (Product # 13-8813-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

The transcriptomes of novel marmoset monkey embryonic stem cell lines reflect distinct genomic features.

Scientific reports

Debowski K,Drummer C,Lentes J,Cors M,Dressel R,Lingner T,Salinas-Riester G,Fuchs S,Sasaki E,Behr R

Published figure using SSEA1 monoclonal antibody (Product # 13-8813-82) in Immunohistochemistry

Thu Jul 07 00:00:00 EDT 2016

The transcriptomes of novel marmoset monkey embryonic stem cell lines reflect distinct genomic features.

Scientific reports

Debowski K,Drummer C,Lentes J,Cors M,Dressel R,Lingner T,Salinas-Riester G,Fuchs S,Sasaki E,Behr R

Published figure using SSEA1 monoclonal antibody (Product # 13-8813-82) in Immunohistochemistry

Thu Jul 07 00:00:00 EDT 2016

Plant Homeo Domain Finger Protein 8 Regulates Mesodermal and Cardiac Differentiation of Embryonic Stem Cells Through Mediating the Histone Demethylation of pmaip1.

Stem cells (Dayton, Ohio)

Tang Y,Hong YZ,Bai HJ,Wu Q,Chen CD,Lang JY,Boheler KR,Yang HT

13881382 was used in immunocytochemistry to study the role of PHF8 during mesodermal and cardiac lineage commitment using mouse embryonic stem cells

Wed Jun 01 00:00:00 EDT 2016

Alternative Routes to Induced Pluripotent Stem Cells Revealed by Reprogramming of the Neural Lineage.

Stem cell reports

Jackson SA,Olufs ZP,Tran KA,Zaidan NZ,Sridharan R

Published figure using SSEA1 monoclonal antibody (Product # 13-8813-82) in Immunofluorescence

Tue Mar 08 00:00:00 EST 2016

NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers.

Nature

Murakami K,Günesdogan U,Zylicz JJ,Tang WWC,Sengupta R,Kobayashi T,Kim S,Butler R,Dietmann S,Surani MA

Published figure using SSEA1 monoclonal antibody (Product # 13-8813-82) in Flow Cytometry

Thu Jan 21 00:00:00 EST 2016

Parent stem cells can serve as niches for their daughter cells.

Nature

Pardo-Saganta A,Tata PR,Law BM,Saez B,Chow RD,Prabhu M,Gridley T,Rajagopal J

Published figure using SSEA1 monoclonal antibody (Product # 13-8813-82) in Flow Cytometry

Thu Jul 30 00:00:00 EDT 2015

Parent stem cells can serve as niches for their daughter cells.

Nature

Pardo-Saganta A,Tata PR,Law BM,Saez B,Chow RD,Prabhu M,Gridley T,Rajagopal J

Published figure using SSEA1 monoclonal antibody (Product # 13-8813-82) in Flow Cytometry

Thu Jul 30 00:00:00 EDT 2015

Parent stem cells can serve as niches for their daughter cells.

Nature

Pardo-Saganta A,Tata PR,Law BM,Saez B,Chow RD,Prabhu M,Gridley T,Rajagopal J

Published figure using SSEA1 monoclonal antibody (Product # 13-8813-82) in Flow Cytometry

Thu Jul 30 00:00:00 EDT 2015

Coexpression analysis of CD133 and CD44 identifies proneural and mesenchymal subtypes of glioblastoma multiforme.

Oncotarget

Brown DV,Daniel PM,D'Abaco GM,Gogos A,Ng W,Morokoff AP,Mantamadiotis T

13-8813 was used in Flow cytometry/Cell sorting to identify two new potential protein markers for molecular glioma subtype.

Fri Mar 20 00:00:00 EDT 2015

Injury induces direct lineage segregation of functionally distinct airway basal stem/progenitor cell subpopulations.

Cell stem cell

Pardo-Saganta A,Law BM,Tata PR,Villoria J,Saez B,Mou H,Zhao R,Rajagopal J

Published figure using SSEA1 monoclonal antibody (Product # 13-8813-82) in Immunofluorescence

Thu Feb 05 00:00:00 EST 2015

Combinatorial modulation of signaling pathways reveals cell-type-specific requirements for highly efficient and synchronous iPSC reprogramming.

Stem cell reports

Vidal SE,Amlani B,Chen T,Tsirigos A,Stadtfeld M

13-8813 was used in Immunocytochemistry to define cell-type-specific requirements for the rapid and synchronous reprogramming of somatic cells.

Tue Oct 14 00:00:00 EDT 2014

Dax1 and Nanog act in parallel to stabilize mouse embryonic stem cells and induced pluripotency.

Nature communications

Zhang J,Liu G,Ruan Y,Wang J,Zhao K,Wan Y,Liu B,Zheng H,Peng T,Wu W,He P,Hu FQ,Jian R

Published figure using SSEA1 monoclonal antibody (Product # 13-8813-82) in Flow Cytometry

Mon Oct 06 00:00:00 EDT 2014

Distinct and combinatorial functions of Jmjd2b/Kdm4b and Jmjd2c/Kdm4c in mouse embryonic stem cell identity.

Molecular cell

Das PP,Shao Z,Beyaz S,Apostolou E,Pinello L,De Los Angeles A,O'Brien K,Atsma JM,Fujiwara Y,Nguyen M,Ljuboja D,Guo G,Woo A,Yuan GC,Onder T,Daley G,Hochedlinger K,Kim J,Orkin SH

Published figure using SSEA1 monoclonal antibody (Product # 13-8813-82) in Flow Cytometry

Thu Jan 09 00:00:00 EST 2014

Cell reprogramming requires silencing of a core subset of polycomb targets.

PLoS genetics

Fragola G,Germain PL,Laise P,Cuomo A,Blasimme A,Gross F,Signaroldi E,Bucci G,Sommer C,Pruneri G,Mazzarol G,Bonaldi T,Mostoslavsky G,Casola S,Testa G

Published figure using SSEA1 monoclonal antibody (Product # 13-8813-82) in Flow Cytometry

Thu Jun 13 00:00:00 EDT 2013

Cell reprogramming requires silencing of a core subset of polycomb targets.

PLoS genetics

Fragola G,Germain PL,Laise P,Cuomo A,Blasimme A,Gross F,Signaroldi E,Bucci G,Sommer C,Pruneri G,Mazzarol G,Bonaldi T,Mostoslavsky G,Casola S,Testa G

Published figure using SSEA1 monoclonal antibody (Product # 13-8813-82) in Flow Cytometry

Thu Jun 13 00:00:00 EDT 2013

Cell reprogramming requires silencing of a core subset of polycomb targets.

PLoS genetics

Fragola G,Germain PL,Laise P,Cuomo A,Blasimme A,Gross F,Signaroldi E,Bucci G,Sommer C,Pruneri G,Mazzarol G,Bonaldi T,Mostoslavsky G,Casola S,Testa G

Published figure using SSEA1 monoclonal antibody (Product # 13-8813-82) in Flow Cytometry

Thu Jun 13 00:00:00 EDT 2013

In vitro transformation of mouse testis cells by oncogene transfection.

Biology of reproduction

Morimoto H,Lee J,Tanaka T,Ishii K,Toyokuni S,Kanatsu-Shinohara M,Shinohara T

13-8813 was used in Flow cytometry/Cell sorting to develop a novel strategy to transform mouse testis cells in vitro for the study of germ cell tumours.

Tue May 01 00:00:00 EDT 2012

Embryonic stem cells require Wnt proteins to prevent differentiation to epiblast stem cells.

Nature cell biology

ten Berge D,Kurek D,Blauwkamp T,Koole W,Maas A,Eroglu E,Siu RK,Nusse R

Published figure using SSEA1 monoclonal antibody (Product # 13-8813-82) in Flow Cytometry

Sun Aug 14 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: 3-fucosyl-N-acetyl-lactosamine epitope; 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; alpha (1,3) fucosyltransferase, myeloid specific; alpha (1,3) fucosyltransferase, myeloid-specific; alpha 1,3-fucosyltransferase; Alpha-(1,3)-fucosyltransferase 4; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; Fuc-TIV; Fucosyltransferase 4; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase IV; fucT-IV; Galactoside 3-L-fucosyltransferase; Lewis X; stage-specific embryonic antigen 1

Gene Aliases: AI451562; CD15; ELFT; FAL; FCT3A; FUC-TIV; FucT-IV; FUT4; FUTIV; LeX; SSEA-1; Ssea1

UniProt ID: (Human) P22083, (Mouse) Q11127

Entrez Gene ID: (Human) 2526, (Mouse) 14345

Molecular Function: glycosyltransferase

Function(s)

carbohydrate metabolic process protein glycosylation protein N-linked glycosylation fucosylation L-fucose catabolic process

Process(es)

SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), Biotin, eBioscience™

Product Details

13-8813-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (FN)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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