Invitrogen

SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), Biotin, eBioscience™

Product Image Gallery

SSEA4 Antibody in Flow Cytometry (Flow) — Staining of 2102ep cell line with staining buffer (autofluorescence) (open histogram) or 0.5 µg of Anti-Human SSEA-4 Biotin (filled histogram). Total viable cells were used for analysis.

SSEA4 Antibody in Immunocytochemistry (ICC/IF) — Figure 3. SIMR Fibroblasts Can Be Reprogrammed (A) Native BJ and SIMR fibroblasts reprogrammed to iPSCs with TRA-1-60 + clones counted by microscopy. Data are the means of biological duplicates. Data for BJ fibroblast control are the same data as in Figure S3A . (B) Flow cytometry of pluripotency biomarkers SOX2 and OCT3/4, and fibroblast biomarker CD44. Immunostained samples are shown in color with isotype negative controls in gray. Representative data for native BJ fibroblasts and BJ-iPSCs, and for BJ rho0+PBMC1-iPSC cells. Data for the native BJ fibroblasts and BJ-iPSCs shown here are the same as in Figure S3B . (C) Representative phase contrast and IF microscopy images of native BJ fibroblast (negative control), BJ-iPSC (positive control), and three BJ rho0+PBMC1-iPSC clones immunostained for pluripotency biomarkers SSEA-4 and OCT4. Scale bars, 100 mum. (D) OCR measurements for ~1.5 x 10 4 native BJ-iPSCs and BJ rho0+PBMC1-iPSC clones 1, 2, and 11. Data for BJ-iPSC control are the same as in Figure S3D . Data are the means +- SD of four technical replicates. Statistical significance by unpaired, two-tailed Student''s t test. *p 0.05; **p 0.01 See also Figure S1 and Table S2 .

SSEA4 Antibody in Immunohistochemistry (IHC) — FIG. 3. Expression of fetal cardiac stem cell markers ISL1, WT1, SSEA4, and cardiomyocyte marker cTnT, in the donor AVj (a) A representative photo of IHC staining with antibodies against ISl1 ( yellow ), WT1 ( red ), and cTnT ( green ) showing the clusters of WT1 + /ISl1 + /cTnT + cells at the border to myocardium ( arrowheads ). (aI-III) Further enlargement of the region within the dashed line in (a) show co-expression of WT1 + /ISl1 + /cTnT + cells ( arrows ). (b) A representative photo of IHC staining with antibodies against SSEA4 ( yellow ) and cTnT ( green ) showing a streak of SSEA4 + cells in the connective tissue region. (bI-II) Further enlargement of the border to myocardium shows SSEA4 + /cTnT + cells, in this region.

Product Details

13-8843-82

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	1 µg/test	View 3 publications 3 publications

Product Specifications
Species Reactivity	Dog, Chicken, Human, Mouse, Non-human primate, Rabbit
Published species	Not Applicable
Host/Isotype	Mouse / IgG3
Class	Monoclonal
Type	Antibody
Clone	eBioMC-813-70 (MC-813-70)
Conjugate	Biotin Alexa Fluor 488 eFluor 570 eFluor 660 PE PerCP-eFluor 710 Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_657850

Product Specific Information

Description: The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein. Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), human induced pluripotent stem (iPS) cells, and human embryonic stem cells (ES) with a decrease in expression upon differentiation. Recently expression has also been found on bone marrow mesenchymal stem cells.

The eBioMC-813-70 antibody has been reported to crossreact to mouse, rabbit, canine, chicken, and Old and New World monkeys.

Applications Reported: This eBioMC-813-70 (MC-813-70) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioMC-813-70 (MC-813-70) antibody has been tested by flow cytometric analysis of the 2102Ep cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pressure-Driven Mitochondrial Transfer Pipeline Generates Mammalian Cells of Desired Genetic Combinations and Fates.

Cell reports

Patananan AN,Sercel AJ,Wu TH,Ahsan FM,Torres A,Kennedy SAL,Vandiver A,Collier AJ,Mehrabi A,Van Lew J,Zakin L,Rodriguez N,Sixto M,Tadros W,Lazar A,Sieling PA,Nguyen TL,Dawson ER,Braas D,Golovato J,Cisneros L,Vaske C,Plath K,Rabizadeh S,Niazi KR,Chiou PY,Teitell MA

Published figure using SSEA4 monoclonal antibody (Product # 13-8843-82) in Immunocytochemistry

Tue Dec 29 00:00:00 EST 2020

The Atrioventricular Junction: A Potential Niche Region for Progenitor Cells in the Adult Human Heart.

Stem cells and development

Vukusic K,Sandstedt M,Jonsson M,Jansson M,Oldfors A,Jeppsson A,Dellgren G,Lindahl A,Sandstedt J

Published figure using SSEA4 monoclonal antibody (Product # 13-8843-82) in Immunofluorescence

Thu Aug 15 00:00:00 EDT 2019

An induced pluripotent stem cell line (TRNDi006-A) from a MPS IIIB patient carrying homozygous mutation of p.Glu153Lys in the NAGLU gene.

Stem cell research

Huang W,Xu M,Li R,Baskfield A,Kouznetsova J,Beers J,Zou J,Liu C,Zheng W

Published figure using SSEA4 monoclonal antibody (Product # 13-8843-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2019

Generation of an induced pluripotent stem cell line (TRNDi008-A) from a Hunter syndrome patient carrying a hemizygous 208insC mutation in the IDS gene.

Stem cell research

Hong J,Xu M,Li R,Cheng YS,Kouznetsova J,Beers J,Liu C,Zou J,Zheng W

Published figure using SSEA4 monoclonal antibody (Product # 13-8843-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2019

Simplified Footprint-Free Cas9/CRISPR Editing of Cardiac-Associated Genes in Human Pluripotent Stem Cells.

Stem cells and development

Kondrashov A,Duc Hoang M,Smith JGW,Bhagwan JR,Duncan G,Mosqueira D,Munoz MB,Vo NTN,Denning C

Published figure using SSEA4 monoclonal antibody (Product # 13-8843-82) in Flow Cytometry

Thu Mar 15 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: SSEA-4; stage-specific embryonic antigen 4

SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), Biotin, eBioscience™

Product Details

13-8843-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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