Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Flow Cytometry (Flow) |
1 µg/test | View 3 publications 3 publications |
Immunocytochemistry (ICC/IF) |
- | View 1 publication 1 publication |
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Dog, Chicken, Human, Mouse, Non-human primate, Rabbit |
Published species |
Not Applicable |
Host/Isotype |
Mouse / IgG3 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
eBioMC-813-70 (MC-813-70) |
Conjugate |
Biotin
|
Form |
Liquid |
Concentration |
0.5 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.2 |
Contains |
0.09% sodium azide |
Storage conditions |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_657850 |
Description: The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein. Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), human induced pluripotent stem (iPS) cells, and human embryonic stem cells (ES) with a decrease in expression upon differentiation. Recently expression has also been found on bone marrow mesenchymal stem cells.
The eBioMC-813-70 antibody has been reported to crossreact to mouse, rabbit, canine, chicken, and Old and New World monkeys.
Applications Reported: This eBioMC-813-70 (MC-813-70) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioMC-813-70 (MC-813-70) antibody has been tested by flow cytometric analysis of the 2102Ep cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: SSEA-4; stage-specific embryonic antigen 4
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