Invitrogen

SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), eFluor™ 570, eBioscience™

Product Image Gallery

SSEA4 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry on fixed NTERA-2 cells using 10 µg/mL Anti-Human SSEA-4 eFluor® 570. Nuclei are stained with DAPI.

SSEA4 Antibody in Immunohistochemistry (IHC) — FIG. 3. Expression of fetal cardiac stem cell markers ISL1, WT1, SSEA4, and cardiomyocyte marker cTnT, in the donor AVj (a) A representative photo of IHC staining with antibodies against ISl1 ( yellow ), WT1 ( red ), and cTnT ( green ) showing the clusters of WT1 + /ISl1 + /cTnT + cells at the border to myocardium ( arrowheads ). (aI-III) Further enlargement of the region within the dashed line in (a) show co-expression of WT1 + /ISl1 + /cTnT + cells ( arrows ). (b) A representative photo of IHC staining with antibodies against SSEA4 ( yellow ) and cTnT ( green ) showing a streak of SSEA4 + cells in the connective tissue region. (bI-II) Further enlargement of the border to myocardium shows SSEA4 + /cTnT + cells, in this region.

Product Details

41-8843-82

Applications	Tested Dilution	Publications
Immunocytochemistry (ICC/IF)	10 µg/mL	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Dog, Chicken, Human, Mouse, Non-human primate, Rabbit
Published species	Not Applicable
Host/Isotype	Mouse / IgG3
Class	Monoclonal
Type	Antibody
Clone	eBioMC-813-70 (MC-813-70)
Conjugate	eFluor™ 570 Alexa Fluor 488 Biotin eFluor 660 PE PerCP-eFluor 710 Unconjugated
Excitation/Emission Max	556/569 nm View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_11217876

Product Specific Information

Description: The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein. Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), human induced pluripotent stem (iPS) cells, and human embryonic stem cells (ES) with a decrease in expression upon differentiation. Recently expression has also been found on bone marrow mesenchymal stem cells.

The eBioMC-813-70 antibody has been reported to crossreact to mouse, rabbit, canine, chicken, and Old and New World monkeys.

Applications Reported: This eBioMC-813-70 (MC-813-70) antibody has been reported for use in immunocytochemistry. This product has not been validated for flow cytometric analysis.

Applications Tested: This eBioMC-813-70 (MC-813-70) antibody has been tested by immunocytochemistry on fixed NTERA-2 and 2102Ep cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.

Excitation: 555 nm; Emission: 570 nm

Target Information

SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pressure-Driven Mitochondrial Transfer Pipeline Generates Mammalian Cells of Desired Genetic Combinations and Fates.

Cell reports

Patananan AN,Sercel AJ,Wu TH,Ahsan FM,Torres A,Kennedy SAL,Vandiver A,Collier AJ,Mehrabi A,Van Lew J,Zakin L,Rodriguez N,Sixto M,Tadros W,Lazar A,Sieling PA,Nguyen TL,Dawson ER,Braas D,Golovato J,Cisneros L,Vaske C,Plath K,Rabizadeh S,Niazi KR,Chiou PY,Teitell MA

Published figure using SSEA4 monoclonal antibody (Product # 41-8843-82) in Immunocytochemistry

Tue Dec 29 00:00:00 EST 2020

The Atrioventricular Junction: A Potential Niche Region for Progenitor Cells in the Adult Human Heart.

Stem cells and development

Vukusic K,Sandstedt M,Jonsson M,Jansson M,Oldfors A,Jeppsson A,Dellgren G,Lindahl A,Sandstedt J

Published figure using SSEA4 monoclonal antibody (Product # 41-8843-82) in Immunofluorescence

Thu Aug 15 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: SSEA-4; stage-specific embryonic antigen 4

SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), eFluor™ 570, eBioscience™

Product Details

41-8843-82

Immunocytochemistry (ICC/IF)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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