|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (36 CPRQGKPSYTVDWYYSQT 53) of ST2/IL1RL1 protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||4.0-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
ST2 is a membrane protein belonging to the interleukin-1 receptor family with three Ig-like C2-type (immunoglobulin-like) domains and a TIR domain. ST2, also known as IL1RL1, is a receptor for interleukin-33 (IL-33) which upon stimulation recruits the signaling molecules MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Reports also suggest its possible role in helper T-cell function as well as in cell growth. Three isoforms of ST2 have been reported where isoform C is a membrane form and isoform B is a secreted form. ST2 may be induced by proinflammatory stimuli, and may also be found in serum of autoimmune disease. Different isoforms are restricted to different tissues while high expression of this protein is evident in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Spinal IL-33/ST2 Signaling Contributes to Neuropathic Pain via Neuronal CaMKII-CREB and Astroglial JAK2-STAT3 Cascades in Mice.
PA5-23316 was used in immunohistochemistry - frozen section and western blot to determine the correlation between neuronal caMKII-CREB and astroglial JAK2-STAT3 cascades in mice and how spinal IL-33/ST2 signaling contributes to neuropathic pain
|Liu S,Mi WL,Li Q,Zhang MT,Han P,Hu S,Mao-Ying QL,Wang YQ||Anesthesiology (123:1154)||2015|