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Immunofluorescence analysis of STAT3 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with STAT3 Mouse Monoclonal Antibody (137000) at 2 ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||A GST-fusion protein containing the unique carboxy terminal domain of the murine STAT3 protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay Dependent|
|ELISA (ELISA)||Assay Dependent|
|Gel Shift (GS)||Assay Dependent|
|Immunocytochemistry (ICC)||2-3 µg/ml|
|Immunofluorescence (IF)||2-3 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT1 and STAT2) which were involved in interferon-alpha (IFN-alpha) and IFN-gamma signal transduction. Since then, several additional STAT proteins have been identified (STAT3, 4, 5a, 5b, and 6). STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. In some cases this process is mediated by JAK Kinases (Janus Kinases 1, 2, and 3). For maximum activation of these proteins, phosphorylation at specific tyrosine and serine residues may be required in STAT1 alpha, 3, 4, and 5. Specific functions of the various members of the STAT family are poorly understood. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Dysregulated STAT1-SOCS1 control of JAK2 promotes mammary luminal progenitor cell survival and drives ERα(+) tumorigenesis.
13-7000 was used in EMSA to investigate the role of STAT1 in mammary luminal progenitor cell survival and tumorigenesis
|Chan SR,Rickert CG,Vermi W,Sheehan KC,Arthur C,Allen JA,White JM,Archambault J,Lonardi S,McDevitt TM,Bhattacharya D,Lorenzi MV,Allred DC,Schreiber RD||Cell death and differentiation (21:234)||2014|
BLIMP-1 and STAT3 counterregulate microRNA-21 during plasma cell differentiation.
13-7000 was used in western blot to investigate microRNA expression during plasma cell differentiation.
|Barnes NA,Stephenson S,Cocco M,Tooze RM,Doody GM||Journal of immunology (Baltimore, Md. : 1950) (189:253)||2012|
acute phase response factor; acute-phase response factor; APRF; DNA-binding protein APRF; signal transducer and activator of transcription 3; signal transducer and activator of transcription 3 (acute-phase response factor); STAT3
1110034C02Rik; ADMIO; ADMIO1; APRF; AW109958; HIES; STAT3