Western Blot staining of HEK293 lysate overexpressing Human STAT4 (c-term Myc tagged and loaded in Lane A and C) stained with PA5-19272 at a concentration of 1 ug/ml (lane A). Lane C was stained with anti-MYC Tag at a dilution of (1/1000). Lane B was loaded with mock-transfected HEK293 cells and stained using PA5-19272 at a concentration of 1 mg/ml. Primary antibody incubation was performed for 1 hour and detection was performed by chemiluminescence.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide sequence (TLSNRRFVLCGTNVK) corresponding to the internal amino acids of STAT4|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.