|Tested species reactivity||Hamster, Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide derived from an internal region of human STAT5.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
OPA1-03010 detects signal transducer and activator of transcription 5 a/b (STAT5) from human, mouse and hamster tissues and cells. Rat tissues are expected to react based on sequence homology. This antibody is specific for STAT5 and does not cross-react with any other STATs or related proteins.
OPA1-03010 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~92 and ~85 kDa protein representing STAT5a and STAT5b respectively from CTLL cell lysate.
OPA1-03010 was raised against a synthetic peptide derived from an internal region of human STAT5. The sequence of the immunogen is identical in rat.
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Transcriptional regulation of insulin-like growth factor-I by interferon-gamma requires STAT-5b.
OPA1-03010 was used in western blot to investigate the role of interferon-gama in transcriptional regulation of insulin-like growth factor.
|Hwa V,Little B,Kofoed EM,Rosenfeld RG||The Journal of biological chemistry (279:2728)||2004|