Western blot analysis was performed on whole cell extracts (30 ug lysate) of HeLa (Lane 1), K562 (Lane 2), HEL 92.1.7 (Lane 3) and Hep G2 (Lane 4). The blots were probed with Anti-STAT5 Mouse Monoclonal Antibody (Product# 335900, 1-3 ug/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 626520, 1:4000 dilution). A 86 kDa band corresponding to STAT5 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a transferred onto a nitrocellulose membrane with Pierce™ Power Blotter System (22834). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||All, Virus, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||A peptide corresponding to a conserved amino acid sequence found near the carboxy-terminus of murine STAT5. This peptide encompasses the conserved C-terminal tyrosine phosphorylation site (Y-694) of murine STAT5|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||1-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Mouse||Not Cited||Viral FLIP impairs survival of activated T cells and generation of CD8+ T cell memory.||Wu Z,Roberts M,Porter M,Walker F,Wherry EJ,Kelly J,Gadina M,Silva EM,DosReis GA,Lopes MF,O'Shea J,Leonard WJ,Ahmed R,Siegel RM||Journal of immunology (Baltimore, Md. : 1950) (172:6313)||2004|
|Mechanisms of oncogenic KIT signal transduction in primary gastrointestinal stromal tumors (GISTs).||Duensing A,Medeiros F,McConarty B,Joseph NE,Panigrahy D,Singer S,Fletcher CD,Demetri GD,Fletcher JA||Oncogene (23:3999)||2004|
|Not Applicable||Not Cited||
PU.1 supports proliferation of immature erythroid progenitors.
33-5900 was used in western blot to propose that PU.1 has an important role in regulating the proliferation of immature erythroid progenitors
|Fisher RC,Slayton WB,Chien C,Guthrie SM,Bray C,Scott EW||Leukemia research (28:83)||2004|
|Human||Not Cited||The Epstein-Barr virus latent membrane protein 1 putative Janus kinase 3 (JAK3) binding domain does not mediate JAK3 association or activation in B-lymphoma or lymphoblastoid cell lines.||Higuchi M,Kieff E,Izumi KM||Journal of virology (76:455)||2002|