Immunofluorescence analysis of STAT5 alpha was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with STAT5 alpha Mouse Monoclonal Antibody (133600) at 2 µg/mL and incubated for 3 hours at room temperature and then labeled with Alexa Flour 488 Rabbit Anti-Mouse IgG Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing cytoplasmic localization. Panel e shows no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Tag, Rat, Virus, Non-human primate, Bovine, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||A peptide corresponding to the carboxy-terminus of murine Stat5a coupled to a protein carrier.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Gel Shift (GS)||1-3 ug|
|Immunocytochemistry (ICC)||1-3 ug/ml|
|Immunofluorescence (IF)||1-3 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:50|
|Immunoprecipitation (IP)||5 ug|
|Western Blot (WB)||1-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Identification of STAT3 and STAT5 proteins in the rat suprachiasmatic nucleus and the Day/Night difference in astrocytic STAT3 phosphorylation in response to lipopolysaccharide.
13-3600 was used in immunohistochemistry (free floating) to examine the daily rhythm of STAT3 protein.
|Moravcová S,¿ervená K,Pa¿esová D,Bendová Z||Journal of neuroscience research (94:99)||2016|
Prolactin signalling in the mouse hypothalamus is primarily mediated by signal transducer and activator of transcription factor 5b but not 5a.
13-3600 was used in immunohistochemistry - free floating and western blot to report that STAT5b and not STAT5a is the primary mediator prolactin signaling in the hypothalamus.
|Yip SH,Eguchi R,Grattan DR,Bunn SJ||Journal of neuroendocrinology (24:1484)||2012|
DNA methylation and transcription in a distal region upstream from the bovine AlphaS1 casein gene after once or twice daily milking.
13-3600 was used in EMSA to investigate the role of CpG methylation in milk production
|Nguyen M,Boutinaud M,Pétridou B,Gabory A,Pannetier M,Chat S,Bouet S,Jouneau L,Jaffrezic F,Laloë D,Klopp C,Brun N,Kress C,Jammes H,Charlier M,Devinoy E||PloS one (9:null)||2014|
Preoperative irradiation for the prevention of heterotopic ossification induces local inflammation in humans.
13-3600 was used in western blot to test if preoperative irradiation impacts local immune components.
|Hoff P,Rakow A,Gaber T,Hahne M,Sentürk U,Strehl C,Fangradt M,Schmidt-Bleek K,Huscher D,Winkler T,Matziolis D,Matziolis G,Badakhshi H,Burmester GR,Duda GN,Perka C,Buttgereit F||Bone (55:93)||2013|
A novel role of microRNA146b in promoting mammary alveolar progenitor cell maintenance.
13-3600 was used in western blot to demonstrate that miR46b promotes the maintenance of pregnancy-derived mammary luminal alveolar progenitors.
|Elsarraj HS,Hong Y,Valdez K,Carletti M,Salah SM,Raimo M,Taverna D,Prochasson P,Bharadwaj U,Tweardy DJ,Christenson LK,Behbod F||Journal of cell science (126:2446)||2013|
Rac1 signalling modulates a STAT5/BCL-6 transcriptional switch on cell-cycle-associated target gene promoters.
13-3600 was used in western blot to report that Rac signaling controls a group of target genes repressed by BCL-6 and activated by STAT5A.
|Barros P,Lam EW,Jordan P,Matos P||Nucleic acids research (40:7776)||2012|
A humanized pattern of aromatase expression is associated with mammary hyperplasia in mice.
13-3600 was used in western blot to use transgenic humanized aromatase mice to study the link between aromatase expression in mammary adipose tissue and breast pathology.
|Zhao H,Pearson EK,Brooks DC,Coon JS,Chen D,Demura M,Zhang M,Clevenger CV,Xu X,Veenstra TD,Chatterton RT,DeMayo FJ,Bulun SE||Endocrinology (153:2701)||2012|
|Activated signal transducer and activator of transcription (STAT) 3: localization in focal adhesions and function in ovarian cancer cell motility.||Silver DL,Naora H,Liu J,Cheng W,Montell DJ||Cancer research (64:3550)||2004|
|The coactivator of transcription CREB-binding protein interacts preferentially with the glycosylated form of Stat5.||Gewinner C,Hart G,Zachara N,Cole R,Beisenherz-Huss C,Groner B||The Journal of biological chemistry (279:3563)||2004|
|Mouse||Not Cited||Suppressor of cytokine signaling-1 regulates signaling in response to interleukin-2 and other gamma c-dependent cytokines in peripheral T cells.||Cornish AL,Chong MM,Davey GM,Darwiche R,Nicola NA,Hilton DJ,Kay TW,Starr R,Alexander WS||The Journal of biological chemistry (278:22755)||2003|
|Mouse||Not Cited||Suppressor of cytokine signaling-1 is a critical regulator of interleukin-7-dependent CD8+ T cell differentiation.||Chong MM,Cornish AL,Darwiche R,Stanley EG,Purton JF,Godfrey DI,Hilton DJ,Starr R,Alexander WS,Kay TW||Immunity (18:475)||2003|
|Mouse||Not Cited||STAT5-induced Id-1 transcription involves recruitment of HDAC1 and deacetylation of C/EBPbeta.||Xu M,Nie L,Kim SH,Sun XH||The EMBO journal (22:893)||2003|
|Not Applicable||Not Cited||
Integration of prolactin and glucocorticoid signaling at the beta-casein promoter and enhancer by ordered recruitment of specific transcription factors and chromatin modifiers.
13-3600 was used in ChIP assay to examine the effects of prolactin and glucocorticoids on chromatin remodeling and transcriptional activation in mammary epithelial cells
|Kabotyanski EB,Huetter M,Xian W,Rijnkels M,Rosen JM||Molecular endocrinology (Baltimore, Md.) (20:2355)||2006|
||STAT5-induced Id-1 transcription involves recruitment of HDAC1 and deacetylation of C/EBPbeta.||Xu M,Nie L,Kim SH,Sun XH||The EMBO journal (22:893)||2003|
||Autoinhibition of Jak2 tyrosine kinase is dependent on specific regions in its pseudokinase domain.||Saharinen P,Vihinen M,Silvennoinen O||Molecular biology of the cell (14:1448)||2003|
|Non-human primate||Not Cited||
Cooperation among Stat1, glucocorticoid receptor, and PU.1 in transcriptional activation of the high-affinity Fc gamma receptor I in monocytes.
13-3600 was used in immunoprecipitation and western blot to characterize the functional cooperation between IFN-gamma and dexamethasone in the induction of the high-affinity Fc gamma receptor I in monocytes.
|Aittomäki S,Pesu M,Groner B,Jänne OA,Palvimo JJ,Silvennoinen O||Journal of immunology (Baltimore, Md. : 1950) (164:5689)||2000|