|ELISA (ELISA)||0.1-1 µg/mL|
|Gel Shift (GS)||1-3 µg|
|Immunocytochemistry (ICC)||1-3 µg/mL|
|Immunofluorescence (IF)||1-3 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:50|
|Immunoprecipitation (IP)||5 µg|
|Western Blot (WB)||1-3 µg/mL|
|Immunohistochemistry - Free Floating (IHC (Free))||See 2 publications below|
|Gel Shift (GS)||See 1 publications below|
|Western Blot (WB)||See 10 publications below|
|ChIP assay (ChIP)||See 2 publications below|
|Immunoprecipitation (IP)||See 2 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Tag , Rat , Virus , Non-human primate , Bovine , Mouse , Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||A peptide corresponding to the C-terminus of murine Stat5a coupled to a protein carrier.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
This antibody recognizes the STAT5a protein from a variety of species including human, mouse, and rat and it reacts specifically with the STAT5a protein and does not exhibit any cross-reactivity with the highly related STAT5b protein. Cross-reactivity with other endogenous STAT isoforms has not been observed. Reactivity has been confirmed by western blotting using cell lysates derived from human A431 cells (+/- EGF treatment), NIH 3T3 cells, MCF-7 cells, and HeLa cells.
STATs (signal transducers and activators of transcription) were originally identified as two novel DNA-binding proteins (STAT1 and STAT2), which were found to play essential roles in interferon IFN-alpha- and IFN-gamma-regulated gene expression. Following the identification of these first two family members, five additional mammalian STAT proteins were cloned, and characterized including: STAT3, STAT4, STAT5a, STAT5b, and STAT6. All STAT proteins share several conserved structural and functional domains. In unstimulated cells, STAT proteins exist largely in the cytoplasm as latent transcription factors. In response to treatment of target cells with cytokines or in some cases growth factors, specific STATs undergo tyrosine phosphorylation, homo-or heterodimerization, nuclear translocation, and DNA binding, which results in the transcriptional activation of distinct target genes. At least one and oftentimes several STAT proteins are activated in response to all cytokines which utilize cytokine receptor superfamily members. Nevertheless, there is a striking specificity of STAT activation is seen in response to individual cytokines. STAT5 or MGF (mammary growth factor) was originally isolated by purification of tyrosine phosphorylated DNA binding proteins from prolactin-stimulated mammary tissue and from IL-3-stimulated myeloid cells. Subsequently, two highly related but distinct Stat5 genes (Stat5a and Stat5b) were identified in mouse. The amino acid sequences of STAT5a and 5b show ~ 96% sequence similarity, and both proteins are co-expressed in most tissues of both virgin and lactating mice. However, differential accumulation of 5a and 5b mRNAs have been reported for both muscle and mammary tissue. STAT5 has been reported to be activated by multiple cytokines including IL-2, IL-7, IL-9, IL-15, IL-3, IL-5, IL-6, GMCSF, TPO, EPO, GH, and Prolactin (PRL). Both the STAT5a and 5b proteins recognize the GAS (gamma interferoin activating sequence) site TTCNNNGAA, and the preferred DNA-binding consensus site has been identified as TTCC(A>T)GGAA. Western blot analysis of the full-length STAT5a protein isolated from a variety of cultured cells, indicates that the protein migrates with an apparent molecular weight of ~95 kDa. C-terminal truncated forms of the STAT5a protein have also been reported. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/ BCL-X(L), which suggests the anti-apoptotic function of this gene in cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Mammary gland factor; mammary gland factor STAT5A; signal transducer and activator of transcription 5; Signal transducer and activator of transcription 5A; STAT5; STAT5A
Gene Aliases: AA959963; MGF; Mpf; STAT5; STAT5A
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